MorphologyMorphology1026-35432949-2556Eco-Vector10179310.17816/morph.101793Research ArticleCHANGES IN THE EXPRESSION OF GAT1 (GABA TRANSPORTER) IN VENTROLATERAL PART OF THE SOLITARY NUCLEUS IN PRENATAL SEROTONIN DEFICIENCY IN RATKhozhaiL. I.Laboratory of Ontogenesis of the Nervous System; Department of Histology and Embryology n.a. prof. A. G. Knorreastarta0505@mail.ruI. P. Pavlov Institute of PhysiologySt. Petersburg State Pediatric Medical University151220191566151927022022Copyright © 2019, Khozhai L.I.2019Objective - to study the expression of GAT1 (GABA transporter) at the early stages of postnatal development in the ventrolateral part of solitary nucleus in prenatal serotonin deficiency in rat. Material and methods. To reduce the level of endogenous serotonin in the embryonic period, the method of triptofan hydroxylase inhibition by parachlorophenilalane (PCHA) (Sigma, USA) was applied. Primary rabbit polyclonal antibodies (anti-GABA transporter 1; GAТ1) (AbCam, UK) were used to detect GAT-1 transporter. Results. It was shown that from the early neonatal period to the beginning of juvenile age, a gradual increase in GAT1 expression occurred in the processes, neural endings and synaptic structures in lateral and ventral portions of the solitary nucleus in control rats. In the lateral and ventral portions in experimental animals, the expression level of the GAT1 transporter during the first and the second postnatal weeks considerably exceeded that in control; however, by the end of the third week, i.e. by the beginning of juvenile age, it decreased and was significantly lower than the control value. Сonclusions. In the respiratory portions of the solitary nucleus in control rats, a gradual decrease in GAT1 expression occurs in the first three weeks of postnatal development. Deficiency of serotonin in the prenatal period leads to impaired expression of the GAT1 transporter in the early postnatal period. The revealed deviations can lead to a change in the transmission of GABA, which, in turn, can cause an imbalance of inhibitory and excitatory effects in the respiratory center in the early postnatal period and, as a result, to be a basis for the development of respiratory dysfunctions at early age.solitary nucleusserotoninGAT-1 GABA transporterearly postnatal periodядро одиночного путисеротонинGAT (транспортер ГАМК)ранний постнатальный период[Augood S. J., Herbison A. E., Emson P. C. Localization of GAT-1 GABA transporter mRNA in rat striatum: cellular coexpression with GAD67 mRNA, GAD67 immunoreactivity, and parvalbumin mRNA // J. Neurosci. 1995. Vol. 15. Iss. l. P. 865-874.][Bernstein E. M., Quick M. W. Regulation of γ-aminobutyric acid (GABA) transporters by extracellular GABA // J. Biol. Chem. 1999. Vol. 274, № 2. P. 889-895.][Bonham A. C., McCrimmon D. F. Neurons in the discrete region of the nucleus tractus solitarius are reguired for the Breuer-Hering reflex in rat // J. Physiol. 1990. Vol. 427. P. 261-280.][Chan R. K., Sawchenko P. E. Organization and transmitter specificity of medullary neurons activated by sustained hypertension: implications for understanding baroreceptor reflex circuitry // J. Neurosci. 1998. Vol. 18. Iss. 1. P. 371-387.][Danbolt N. C. Glutamate uptake // Prog. Neurobiol. 2001. Vol. 65. Iss. 1. P. 1-105.][Isaacson J. S. Spillover in the spotlight // Curr. Biol. 2000. Vol. 10. Iss. 13. P. 475-477.][Isaacson J. S., Solis J. M., Nicoll R. A. Local and diffuse synaptic actions of GABA in the hippocampus // Neuron. 1993. Vol. 10. Iss. 2. P. 165-175.][Fuhrer T. E., Palpagama T. H., Waldvogel H. J., Synek B. J.L., Turner C., Faull R. L., Kwakowsky A. Impaired expression of GABA transporters in the human Alzheimer’s disease hippocampus, subiculum, entorhinal cortex and superior temporal gyrus // Neuroscience. 2017. Vol. 351. P. 108-118.][Gadea A., Lopez-Colome A. M. Glial transporters for glutamate, glycine, and GABA: II. GABA transporters // J. Neurosci. Res. 2001. Vol. 63. Iss. 6. P. 461-468.][Guthmann A., Fritschy J. M., Ottersen O. P., Torp R., Herbert H. GABA, GABA transporters, GABA(A) receptor subunits, and GAD mRNAs in the rat parabrachial and Kölliker-Fuse nuclei // J. Comp. Neurol. 1998. Vol. 400, № 2. P. 229-243.][Liu Q., Wong-Riley M. T. Postnatal changes in the expressions of serotonin 1A, 1B, and 2A receptors in ten brain stem nuclei of the rat: implication for a sensitive period // Neuroscience. 2010. Vol. 165. Iss. 1. P. 61-78.][Kuwana S., Okada Y., Sugawara Y. et. al. Disturbance of neural respiratory control in neonatal mice lacking gaba synthesizing enzyme 67-kda isoform of glutamic acid decarboxylase // Neuroscience. 2003. Vol. 120. Iss. 3. P. 861-870.][Paxinos G., Watson C. The Rat Brain in stereotaxic coordinates. 4th Edition. London: Academic Press, 2012. 420 p.][Serrats J., Mengod G., Cortes R. Expression of serotonin 5-HT2C receptors in GABAergic cells of the anterior raphe nuclei // J. Chem. Neuroanat. 2005. Vol. 29. Iss. 2. P. 83-91.]