MorphologyMorphology1026-35432949-2556Eco-Vector10182210.34922/AE.2020.158.4.009Research ArticlePROLONGED BAKTERIOPHAGAL INFECTION OF GUT MICROBIOTA REDUCES THE EXPRESSION OF ALPHA-SYNUCLEIN IN THE RAT PANETH CELLSSergeyevaT. N.Department of Physiology, Cell Biology and Biotechnologytnbio@ya.ruNikolenkoV. N.Department of Human Anatomy; Department of Normal and Topographical Anatomyvn.nikolenko@ya.ruKuznetsovaI. N.Department of Physiology, Cell Biology and Biotechnology1998.kuznetsova.julia@gmail.comSergeyevV. G.Department of Physiology, Cell Biology and Biotechnology; Educational and Research Laboratorycellbio@ya.ruUdmurt State UniversityI. M. Sechenov First Moscow State University (Sechenov University)Lomonosov Moscow State UniversityIzhevsk State Medical Academy151020201584-5606527022022Copyright © 2020, Sergeyeva T.N., Nikolenko V.N., Kuznetsova I.N., Sergeyev V.G.2020Objective - to investigate the intensity of the alpha - synuclein expression by Paneth cells of rat in normal conditions and prolonged bacteriophagal infection of gut microbiota. Material and methods. Experimental studies were performed on 12 adult Wistar male rats weighting 280-320 g. The rats of the main group (n=7) received rectally a 0,5 ml of solution containing a mixture of bacteriophages directed against 14 human pathogens (Microgen, Russia). The solution was introduced weekly for a period of 12 weeks. Each dose of bacteriophagal mixture contained 0,5×106 units/ml of each phage. Animals of the control group (n=5) received 0,5 ml of sterile physiological saline according to the same scheme. After transcardial perfusion, specimens of proximal portion of ileum 1-2 cm upstream the ileocecal junction were obtained. Serial cryostat sections were used for hematoxylin and eosin staining and for detection of immunopositive alpha-synuclein by monoclonal mouse antibodies (Sigma-Aldrich, USA) and commercially available kit containing avidin-biotin-peroxidase complex (АBC Elite; Vector Laboratories, Burlingame, CA). Results. Prolonged bacteriophage infection led to a significant decrease in the number of alpha-synuclein immunopositive cells compared with control. The area of Paneth cells occupied by the alpha synuclein-immunoreactive product and acidophilic granules significantly reduced. Conclusions. Immunopositive alpha-synuclein was detected in the apical parts of Paneth cells, in the area of acidophilic granules localization. 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