MorphologyMorphology1026-35432949-2556Eco-Vector10185810.17816/morph.101858Research ArticleNEUROPEPTIDE Y-ERGIC NEURONS OF THE SMALL INTESTINE IN POSTNATAL ONTOGENESISMaslyukovP. M.Department of Normal Physiology-VyshnyakovaP. A.Department of Normal Physiology-AryaevaD. A.Department of Normal Physiology-BudnikA. F.Department of Normal and Pathological Anatomy-Yaroslavl’ State Medical University151020191565555827022022Copyright © 2019, Maslyukov P.M., Vyshnyakova P.A., Aryaeva D.A., Budnik A.F.2019Objective - to demonstrate the localization, percentage and morphometric characteristics of NPY-immunoreactive (IR) neurons in the intramural ganglia of the myenteric (MP) and submucous neural plexus (SP) of the small intestine in rats of different age groups. Material and methods. The work was performed on 1-, 10-, 20-, 30-, 60-day-old and 2-year-old rats using immunohistochemical methods. Results. NPY-IR neurons were detected in the small intestine from the moment of birth and throughout all studied age periods. In the intramural ganglia of the MP, their number was very small and did not change significantly during ontogenesis. In the SP, NPY-IR neurons were detected in large numbers in newborns; the maximal number of the neurons was observed at 20-30 days. The average cross-sectional area of the NPY-IR cells in the SP was significantly larger than such area of immunonegative neurons in 10-day-old and older rats. Conclusions. Thus, neuropeptide Y is fairly common in various parts of the autonomic nervous system, including sympathetic, parasympathetic and metasympathetic, and plays an important role in the processes of age-related neuronal development.neuropeptide Yautonomic nervous systementeric gangliasmall intestineontogenesisнейропептид Yавтономная нервная системаинтрамуральные ганглиитонкая кишкаонтогенез[Маслюков П. М., Будник А. Ф., Ноздрачев А. Д. Нейрохимические особенности узлов метасимпатической системы в онтогенезе // Успехи геронтологии. 2017. Т. 30, № 3. С. 347-355.][Ноздрачев А. Д., Маслюков П. М. Нейропептид Y и автономная нервная система // Журн. эволюцион. биохим. и физиол. 2011. Т. 47, № 2. С. 105-112.][Czarnecka M., Lu C., Pons J., Maheswaran I., Ciborowski P., Zhang L., Cheema A., Kitlinska J. Neuropeptide Y receptor interactions regulate its mitogenic activity // Neuropeptides. 2019. Vol. 73. P. 11-24. doi: 10.1016/j.npep.2018.11.008][Chandrasekharan B., Nezami B. G., Srinivasan S. Emerging neuropeptide targets in inflammation: NPY and VIP // Am. J. Physiol. Gastrointest. Liver Physiol. 2013. Vol. 304, № 11. G949-G957.][Cox H. M. Neuropeptide Y receptors; antisecretory control of intestinal epithelial function // Auton. Neurosci. 2007. Vol. 133, № 1. P. 76-85.][Furness J. B. The enteric nervous system. Oxford: Blackwell Publishing, 2006.][Hodges G. J., Jackson D. N., Mattar L., Johnson J. M., Shoemaker J. K. Neuropeptide Y and neurovascular control in skeletal muscle and skin // Am. J. Physiol. Regul. Integr. Comp. Physiol. 2009. Vol. 297, № 3. R546-555.][Masliukov P. M., Emanuilov A. I., Moiseev K., Nozdrachev A. D., Dobrotvorskaya S., Timmermans J. P. Development of non-catecholaminergic sympathetic neurons in para- and prevertebral ganglia of cats // Int. J. Dev. Neurosci. 2015. Vol. 40. P. 76-84.][Masliukov P. M., Konovalov V. V., Emanuilov A. I., Nozdrachev A. D. Development of neuropeptide Y-containing neurons in sympathetic ganglia of rats // Neuropeptides. 2012. Vol. 46, № 6. P. 345-352.][Masliukov P.M., Moiseev K., Emanuilov A. I., Anikina T.A., Zverev A. A., Nozdrachev A. D. Development of neuropeptide Y-mediated heart innervation in rats // Neuropeptides. 2016. Vol. 55. P. 47-54.][Masliukov P. M., Moiseev K., Budnik A. F., Nozdrachev A. D., Timmermans J.-P. Development of calbindin- and calretininimmunopositive neurons in the enteric ganglia of rats // Cell Mol. Neurobiol. 2017. Vol. 37, № 7. P. 1257-1267.][Mongardi Fantaguzzi C., Thacker M., Chiocchetti R., Furness J. B. Identification of neuron types in the submucosal ganglia of the mouse ileum // Cell Tissue Res. 2009. Vol. 336, № 2. P. 179-189.][Saraf R., Mahmood F., Amir R., Matyal R. Neuropeptide Y is an angiogenic factor in cardiovascular regeneration // Eur. J. Pharmacol. 2016. Vol. 776. P. 64-70.][Tan C. M.J., Green P., Tapoulal N., Lewandowski A. J., Lee-son P., Herring N. The Role of Neuropeptide Y in Cardio vascular Health and Disease // Front. Physiol. 2018 Vol. 9. P. 1281. doi: 10.3389/fphys.2018.01281][Tough I. R., Forbes S., Tolhurst R., Ellis M., Herzog H., Bornstein J. C., Cox H. M. Endogenous peptide YY and neuropeptide Y inhibit colonic ion transport, contractility and transit differentially via Y1 and Y2 receptors // Br. J. Pharmacol. 2011. Vol. 164. P. 471-484.]