MorphologyMorphology1026-35432949-2556Eco-Vector11087110.17816/morph.110871Research ArticleKlebsiella–Candida arthritis model in Wistar ratsDanilovaElena I.<p>MD, Cand. Sci. (Med.), Associate Professor</p>danilowa@list.ruhttps://orcid.org/0000-0003-0910-6525FisenkoAndrey P.<p>MD, Dr. Sci. (Med.), Professor</p>info@nczd.ruhttps://orcid.org/0000-0001-8586-7946StadnikovAleksandr A.<p>Dr. Sci. (Biol.), Professor</p>k_histology@orgma.ruhttps://orcid.org/0000-0002-6107-0534PerunovaNatalya B.<p>MD, Dr. Sci. (Med.), Professor of the Russian Academy of Sciences, Associate Professor</p>icis-ofrc@list.ruhttps://orcid.org/0000-0002-6352-8879IvanovaElena V.<p>MD, Dr. Sci. (Med.), Associate Professor</p>walerewna13@gmail.comhttps://orcid.org/0000-0002-4974-8947ChaynikovaIrina N.<p>MD, Dr. Sci. (Med.), Professor</p>chainicova@yandex.ruhttps://orcid.org/0000-0002-8923-8829ChelpachenkoOlga E.oech57@gmail.comhttps://orcid.org/0000-0002-6719-5805Orenburg State Medical UniversityNational Medical Research Center for Children’s HealthOrenburg Federal Research Center of the Ural Branch of the Russian Academy of Sciences1102202316015101109202208102022Copyright © 2023, Eco-Vector2023<p><strong><em>MATERIAL AND METHODS:</em></strong> This study was carried out on 20 male Wistar rats, of which 15 were injected with a bacterialfungal suspension into the cavity of the hock (tarsus) joint. Specifically, the rats were injected with <em>KlebsiellaCandida</em> suspension (1:1) with a microbial concentration of 10<sup>6</sup> CFU/ml prepared by using the museum strains <em>Klebsiella pneumoniae</em> ICIS-278 and <em>Candida albicans</em> ATCC 24433 under the control of an X-ray apparatus and digitizer FireCR+. Five intact animals served as the control. On day 15 of the experiment, a bacteriological and histological examination of the affected joints was carried out while maintaining topography after euthanasia.</p>
<p><strong><em>RESULTS:</em></strong> In rats infected with the <em>KlebsiellaCandidiasis</em> suspension, pain syndrome and functional changes typical of arthritis were noted on days 23. The bacteriological examination of the affected joints in 75% of rats isolated <em>K. pneumonia</em> cultures that were identical, according to polymerase chain reaction, to the original strain that was part of the bacterialfungal suspension. At the same time, fungi of the genus <em>Candida</em>, which was also used in the experiment to infect animals, were not detected in the affected joints of rats. The established model of <em>KlebsiellaCandida</em> arthritis was characterized by the pathomorphological signs of panarthritis accompanied by the purulent inflammation of all joint structures (synovial membrane, cartilage, and bone tissue) with the involvement of soft periarticular tissues.</p>
<p><strong><em>CONCLUSION: </em></strong>The model of <em>Klebsiella</em> and fungal arthritis presented by this work can be used to test new means of antimicrobial (antibacterial and antifungal) therapies, which are an integral component of the treatment of infection-associated arthritis.</p>experimentratsKlebsiella–Candida arthritisэксперименткрысыклебсиеллёзно-кандидозный артрит[Bariteau JT, Waryasz GR, McDonnell M, et al. Fungal osteomyelitis and septic arthritis. J Am Acad Orthop Surg. 2014;22(6):390–401. doi: 10.5435/JAAOS-22-06-390][Laurence M, Asquith M, Rosenbaum JT. Spondyloarthritis, acute anterior uveitis, and fungi: updating the Catterall-King hypothesis. Front Med (Lausanne). 2018;5(80):1–17. doi: 10.3389/fmed.2018.00080][Bukharin OV, Perunova NB. Microsymbiocenosis. Ekaterinburg: UrO RAN; 2014. 260 p. (In Russ).][Krylov PA, Nesmeyanova EN, Terpilovskiy AA, Novochadov VV. Morphology of tibiofemoral joint in rats with experimental osteoarthritis: 3D-reconstruction, based on the technology of high-precision grinding. Journal of Anatomy and Histopathology. 2017;6(3):44–49. (In Russ). doi: 10.18499/2225-7357-2017-6-3-44-49][Muzhikyan AA, Shekunova EV, Kashkin VV, et al. Histological assessment of pathological changes in the joints in different ways of induction of chronic arthritis in rats. Laboratory animals for scientific research. 2018;(1):32–45. (In Russ). doi: 10.29296/2618723X-2018-01-04]