Objective -to study the effect of metabolites from the culture of Bacillus subtilis 804 on the reparative histogenesis in rats during the healing of deep linear skin wounds. Materials and methods. Linear 3.0 cm-long full thickness (extending to the hypodermis) skin wounds were inflicted to 40 Wistar rats. In the experimental group, the wounds were irrigated with a solution of the metabolites of Bacillus subtilis 804 culture, while in the control group they were treated with NaCl isotonic solution. Histochemical and morphometric methods were applied; at Days 3, 7 and 11 the microvessel, collagen fiber, amorphous substance, epidermal and dermal cell volume density was measured. Results. In the experimental group, the earlier migration of macrophages to the damaged region was observed, as well as accelerated proliferation and differentiation of epithelial cells and fibroblasts, accompanied by replacement of type III collagen by type I. Conclusions. Metabolites of Bacillus subtilis 804 optimize the functions of effector cells of the reparative process, which promote the accelerated wound healing.