MorphologyMorphologyМорфология1026-35432949-2556Eco-Vector39939010.17816/morph.399390ArticlesСтатьиMORPHOLOGICAL ANALYSIS OF THE RESULTS OF INJECTION OF THE AUTOLOGOUS STROMAL MARROW-DERIVED STEM CELLS INTO THE UTERINE SCAR IN RATSМОРФОЛОГИЧЕСКИЙ АНАЛИЗ РЕЗУЛЬТАТОВ ВВЕДЕНИЯ АУТОЛОГИЧНЫХ СТВОЛОВЫХ СТРОМАЛЬНЫХ КЛЕТОК КОСТНОМОЗГОВОГО ПРОИСХОЖДЕНИЯ В РУБЕЦ МАТКИ КРЫСMayborodinI VМайбородинИ В

Институт химической биологии и фундаментальной медицины СО РАН

imai@mail.ruYakimovaN VЯкимоваН В

Институт химической биологии и фундаментальной медицины СО РАН

MatveevaV AМатвееваВ А

Институт химической биологии и фундаментальной медицины СО РАН

PekarevO GПекаревО Г

Институт химической биологии и фундаментальной медицины СО РАН

MayborodinaE IМайбородинаЕ И

Институт химической биологии и фундаментальной медицины СО РАН

PekarevaE OПекареваЕ О

Институт химической биологии и фундаментальной медицины СО РАН

TkachukO KТкачукО К

Институт химической биологии и фундаментальной медицины СО РАН

MaiborodinI VYakimovaN VMatveyevaV APekarevO GPekarevaYeI YeOTkachiukO KИнститут химической биологии и фундаментальной медицины СО РАН161220101386NO6 (2010)№6 (2010)475509052023Copyright ©; 2010, Eco-VectorCopyright ©; 2010, Эко-Вектор2010Eco-VectorЭко-Векторhttps://j-morphology.com/1026-3543/article/view/399390

The results of injection of the autologous stromal (mesenchymal) marrow-derived stem cells (ASMSC) transfected with green fluorescent protein (GFP) gene into the rat uterine scar were studied by methods of light microscopy. One week after ASMSC injection into the formed scar on the right side (2 months after the uterine horn ligation), large groups of blood vessels containing the formed elements of blood were found, while these were absent in the uterine scar on the opposite side. The study of the unstained sections in the reflected ultraviolet light demonstrated bright luminescence in the vessel endothelium and adventitia in the uterine horn scar only at the side of ASMSC injection. It is suggested that after the injection of ASMSC in the uterine horn scar, they form blood vessels as result of a differentiation into endotheliocytes and pericytes. The expression of GFP gene not only in vascularl endothelium, but also in their adventitia, indicated that the differentiation ASMSC is possible in both endotheliocytes and in pericytes.

Методами световой микроскопии изучали результаты введения аутологичных стромальных (мезенхимальных) стволовых клеток костномозгового происхождения (АССККП) с трансфицированным геном зеленого флюоресцентного белка (GFP) в рубец маточного рога у крыс. Через 1 нед после введения АССККП в сформированный рубец справа (2 мес после перевязки) в нем присутствовали крупные группы кровеносных сосудов, содержащие форменные элементы крови, которые отсутствовали в рубце рога на противоположной стороне. При исследовании неокрашенных срезов в отраженном ультрафиолетовом свете было обнаружено яркое свечение в эндотелии и наружной оболочке сосудов рубца маточного рога только на стороне инъекции АССККП. Сделано заключение, что после введения в рубец АССККП они формируют кровеносные сосуды за счет дифференцировки в эндотелиоциты и перициты. Экспрессия гена GFP не только в эндотелии сосудов, но и в их наружных оболочках указывает на то, что возможна дифференцировка АССККП как в эндотелиальном, так и в перицитарном направлениях.

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