MorphologyMorphologyМорфология1026-35432949-2556Eco-Vector62903710.17816/morph.629037Original Study ArticlesОригинальные исследованияResearch ArticleСonnexin-43 in cells of injured rat sciatic nerveКоннексин-43 в клетках регенерирующего седалищного нерва крысыhttps://orcid.org/0000-0002-9643-68311479-5992KolosElena A.КолосЕлена Андреевна
Russian Federation
koloselena1984@yandex.ruInstitute of Experimental MedicineИнститут экспериментальной медицины1804202415072023161371781303202412042024Copyright ©; 2023, Eco-VectorCopyright ©; 2023, Эко-Вектор2023Eco-VectorЭко-Векторhttps://eco-vector.com/for_authors.php#07https://j-morphology.com/1026-3543/article/view/629037

BACKGROUND: It is known that intercellular communications in the peripheral nervous system are provided by various types of intercellular contacts, in particular, gap junctions formed by connexin proteins. The literature contains data on changes in the expression of connexin-32, connexin-46 and other types of connexins during nerve injury. However, very few studies describe changes in connexin-43 expression in similar pathologies.

AIM: The purpose of this study was to study the distribution and localization of the gap junction protein connexin-43 (Cx43) in cells of intact and injured rat sciatic nerve.

MATERIALS AND METHODS: Damage to the sciatic nerves of Wistar rats of the experimental group (n=5) was carried out by nerve ligation for 40 s. Animals without damage of sciatic nerve were studied as a control group (n=5). Immunohistochemical detection of Cx43 was performed on paraffin sections.

RESULTS: It has been established that the protein is contained in the cells of the perineurium and epineurium of both the intact nerve and after the application of a ligature. In the area of the endoneurium, in the absence of nerve damage, Cx43 is detected only in the endotheliocytes of a few vessels. In the endoneurium of the injured nerve, a large number of large Cx43-immunopositive cells with processes were identified.

CONCLUSIONS: It has been established that Cx43-containing cells are identified in the endoneurium of the sciatic nerve only after damage. To clarify whether such cells belong to a specific cell type, additional studies are necessary.

Обоснование. Известно, что межклеточные коммуникации в периферической нервной системе обеспечивают различные виды межклеточных контактов, в частности щелевые контакты, сформированные белками коннексинами. В литературе присутствуют данные об изменении экспрессии коннексина-32, коннексина-46 и других типов коннексинов при травме нерва. Однако крайне мало исследований описывают изменение экспрессии коннексина-43 при аналогичной патологии.

Цель исследования — изучить распределение и локализацию белка щелевых контактов коннексина-43 (Сх43) в клетках интактного и повреждённого седалищного нерва крысы.

Материалы и методы. Повреждение седалищных нервов крыс линии Вистар подопытной группы (n=5) было выполнено путём наложения лигатуры в течение 40 с. В качестве группы контроля (n=5) исследованы животные без повреждения седалищного нерва. На парафиновых срезах проводили иммуногистохимическое выявление Сх43.

Результаты. Установлено, что Сх43 содержится в клетках периневрия и эпиневрия как интактного нерва, так и нерва после наложения лигатуры. В области эндоневрия при отсутствии повреждения нерва Сх43 выявляется лишь в эндотелиоцитах немногочисленных сосудов. В эндоневрии повреждённого нерва идентифицируется большое количество крупных отростчатых Cx43-иммунопозитивных клеток.

Заключение. Установлено, что Cx43-содержащие клетки идентифицируются в эндоневрии седалищного нерва только после повреждения. Для уточнения принадлежности таких клеток эндоневрия к определённому клеточному типу необходимы дополнительные исследования.

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