PATHOMORPHOLOGICAL CHANGES OF SPLEEN IN DYNAMICS OF EXPERIMENTAL SEPSIS OF DIFFERENT ETIOLOGIES

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Aim. The comparative morphological studies of spleen in dynamic of experimental sepsis of different etiologies were conducted. Material and Methods. Experiments were carried out on 15 mature chinchila rabbits of both sexes. Sepsis was caused by staphylococcus aureus (I series of experiments) and by simultaneous infection of animals by staphylococcus aureus and staphylococcus epidermidis (II series of experiments); intravenous injection of staphylococcal toxin (0.06 ml) has been performed, which after 48 h was followed by intraperitoneal injection of bacterial culture. Results and Discussion. Spleen tissue was taken after having sacrificed the animals, on 13th and 14th days after bacterial contamination and investigated by histological and morphometric methods. In both series of experiments, on 13th and 14th days after bacterial contamination, the similar increasing changes like microcirculatory disturbances and dystrophic-necrotic changes were observed. Red pulp of the spleen is overflowing with red blood cells; in the sinusoids is observed aggregation of erythrocytes. In spleen tissue are found hemorrhages as well. Were revealed oedema of lymphoid nodules and red pulp, swelling and thickening of the walls of blood vessels, they are infiltrated with blood cells. In some cases boundaries of splenic lymphoid nodules are unclear, poorly distinguished from the red pulp. On 14th day after bacterial contamination, in both series of experiments, mean volume and number of splenic lymphoid nodules are statistically significantly decreased in comparison with the control. By comparison of results of morphometric study of I and II series of experiments no statistically significant differences were detected. The comparison of measured values of rabbit spleen on 13th and 14th days after bacterial contamination did not show any statistically significant differences.