THE MORPHOLOGY OF CEREBRAL CORTEX GANGLIONIC LAYER IN ISCHEMIC STROKE

Abstract

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Aim. To reveal morphofunctional changes in the ganglionic layer of the pyramidal cortex of both hemispheres of the brain of people who died as a result of ischemic stroke. Material and Methods. Samples of cerebral cortex 9 died as a result of left middle cerebral artery ischemic stroke human from 3 areas (1 - adjoining directly to the site of necrosis, 2 - 5-10 cm distant from the previous 3 - the area of the contralateral hemisphere symmetrical hearth) were investigated. Samples were stainedstained with cresyl violetand hematoxylin-eosin; p53 and NSE proteins were detected by indirect immunoperoxidase immunohistochemistry. All samples were examined by light microscopy. Results and Discussion. Morphometric analysis revealed that the number of damaged bodies of neurons of ganglionic cell layer was greatest in the samples of the ipsilateral hemisphere (zone 1 and 2) and minimal in the zone 3. The relative content of reactively altered nerve cells was the smallest in the samples of the contralateral hemisphere. In the zone 2, all neurons were significantly altered, and in the remote from the border region by 5-10 cm, the number of reactively altered neurons significantly prevailed over the unaltered. Hhe highest number of p53 positive pyramidal cells was observed in zone 2; in zone 1 and 3 the number of labeled cells was comparable. Conclusions. With the development of ischemic stroke, quantitative and qualitative changes occur in both hemispheres of the brain. Areas of maximum changes are distributed unevenly.