POSTTRAUMATIC USE OF ERYTHROPOETIN AND LOW DOSE IONISING RADIATION TO PREVENT SECONDARY DAMAGE IN SPINAL CORD TRAUMA

Аннотация

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Background. As clinical outcome of acute traumatic spinal cord injury, many patients are forced to sustain their lives with paralysis. These patients face very difficult medical and social care and many complications over the years. In our study. Aim. İt was planned to use combined treatment modality of erythropoietin and ionizing radiation in the traumatic spinal cord injury model in rats. This study is approved by the Experimental Animal Research Ethical Committee of Marmara University. 60 Sprague Dawey rats were divided into following groups: Trauma 10 Trauma+IR60 10 Trauma+IR200 10 Trauma+EPO 10 Trauma+EPO+IR60 10 Trauma+EPO+IR200 10 Material and Methods. Spinal trauma was created by free fall of 50 g weight on T8-10 segments. Epobel was administered 5000 iu/0.5 ml by iv route 1 hour after the trauma. Ionising radiation was applied at 60 and 200 cGy doses 2 hour after the trauma. Results and Discussion. The results were evaluated by locomotor tests, ultrastructural tissue analysis, Tunel apoptosis test, ependymal stem cell identification, gliosis scores, proliferation analysis, enolase and S100 beta analysis methods. Conclusions. Combination of eritropoetin with low dose raidation is beneficial for protection from secondary effects of spinal cord trauma.