MORPHOLOGICAL JUSTIFICATION OF PLEURAL ADHESION STIMULATION

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Aim. To give the morphological justification of pleural adhesion stimulation. Material and Methods. The chronic pleural empyema was modeled in 290 nonlinear male rats by pleural injection of 1 billion E. coli suspension into the V intercostal space along the axillary line in a volume of 1 ml per week for 8 weeks. The animals of the experimental group were removed 50 mg of adipose tissue from the subcutaneous abdominal fat, followed by homogenization in physiological saline. The resulting suspension was injected into the empyema cavity. The animals of the control group were injected with 500 μl of physiological saline. The animals of both groups were sacrificed on the 30th day of the postoperative period. Results and Discussion. The microscopic examination of pleural adhesions showed the loose connective tissue with areas of leukocyte infiltration with few lymphocytes and macrophages. In the animals of the experimental group, the pleural adhesions were mature and characterized by the predominance of collagen fibers (per cent vol. 27.73±1.39%), while the number of reticular and elastic fibers was limited (per cent vol. 4.01 and 2.73%, respectively). The volume fraction of the vascular bed was 6.27±0.19%. A desolation of capillaries with recalibration of blood vessels was found in the peripheral region. The lymphohysteocyte-predominant infiltration was detected (per cent vol. 23.12±1.29%). Conclusions. Stimulation of the adhesion formation by the pleural injection of the autologous adipose tissue caused the formation of the adhesions filling the empyema cavity. Non identified inflammation indicated to the controlled development of adhesions.