THE ASSOCIATION BETWEEN THE VASCULARIZATION OF SKIN REGENERATE AND THE PROLIFERATIVE ACTIVITY OF ITS CELLS

Using histological, morphometric and immunohistochemical methods, the correlation between the degree of development of the microcirculatory bed and the level of cell proliferative activity was studied in the healing zone of rat full-layer surgical skin wound. The comparison was made between the results obtained during spontaneous course of the reparative process (control group, n = 20) and under the conditions of angiogenesis stimulation by twofold paravulnary injection of 0.1 ml of Neovasculgene pCMV-VEGF165 plasmid encoding endothelial vascular growth factor (experimental group, n = 20). Morphometric analysis of the material obtained on Days 7, 14 and 21 showed that the number of blood vessels in the newly formed granulation tissue in the experimental group was 1.9 times higher than in the control. Immunohistochemical studies have demonstrated the concentration of proliferative processes in the perivascular zone, especially in animals with stimulated angiogenesis. The use of Neovasculgene resulted in the formation of the denser network of microvessels, the increased cell proliferation, the reduction of overall healing times and the formation of organospecific regenerate.

skin, regeneration, stimulated angiogenesis, granulation tissue, proliferation