Transcriptional activity of metallothionein genes in acute poisoning caused by cadmium chloride

Munira M. Ziatdinova; Зиатдинова Мунира Мунировна; Yana V. Valova; Валова Я. В.; Guzel F. Mukhammadiyeva; Мухаммадиева Г. Ф.; Elza N. Usmanova; Усманова Э. Н.; Denis O. Karimov; Каримов Д. О.; Nadezhda Yu. Khusnutdinova; Хуснутдинова Н. Ю.; Samat S. Bajgil’Din; Байгильдин С. С.

doi:10.47470/0016-9900-2020-99-9-990-995

Transcriptional activity of metallothionein genes in acute poisoning caused by cadmium chloride

Authors: Ziatdinova M.M.¹, Valova Y.V.¹, Mukhammadiyeva G.F.¹, Usmanova E.N.¹, Karimov D.O.¹, Khusnutdinova N.Y.¹, Bajgil’Din S.S.¹
Affiliations:
1. Ufa Research Institute of Occupational Health and Human Ecology
Issue: Vol 99, No 9 (2020)
Pages: 990-995
Section: EXPERIMENTAL INVESTIGATIONS
Published: 20.10.2020
URL: https://j-morphology.com/0016-9900/article/view/640268
DOI: https://doi.org/10.47470/0016-9900-2020-99-9-990-995
ID: 640268

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Abstract

Introduction. Cadmium is one of the most toxic heavy metals widely distributed in the environment. It has a long half-life, leading to its accumulation in various organs, which in turn is the cause of many adverse effects on human health.

Material and methods. Acute toxic poisoning with cadmium chloride was simulated on white outbred male rats, divided into groups depending on the exposure time. Samples of liver and kidney tissues were used as study materials, in the homogenate of which the level of mRNA of metallothionein genes was determined.

Results. It was found that the maximum value of the multiplicity of MT1 gene expression in the liver was reached after 6 hours (16.36 ± 0.77; p <0.001), and in the kidneys one day after intoxication with cadmium chloride (6.12 ± 0.43; p <0.001). The activity of the MT2 gene in the liver was most pronounced in the range of 2-4 hours (14.35 ± 1.73; 14.78 ± 1.44; p <0.001), while in the renal tissues an increase in the amount of mRNA was recorded after 24 hours (7.32 ± 0.63; p <0.001). The level of MT3 gene transcripts in liver tissues was decreased throughout the experiment, however, the opposite picture was observed in the kidney tissue, where the maximum was reached one day after the administration of the toxicant (6.14 ± 0.31; p <0.001).

Conclusion. Thus, an increase in metallothionein expression in response to the presence of heavy metal ions in the body can be used as a genetic marker in case of poisoning with various compounds.

Keywords

cadmium chloride, expression, acute intoxication, metallothioneins

About the authors

Munira M. Ziatdinova

Ufa Research Institute of Occupational Health and Human Ecology

Author for correspondence.
Email: munira.munirovna@yandex.ru
ORCID iD: 0000-0002-1848-7959

MD, junior researcher of the Department of Toxicology and Genetics with an experimental laboratory of laboratory animals, Ufa Research Institute of Occupational Health and Human Ecology, Ufa, 450106, Russian Federation.

у-mail: munira.munirovna@yandex.ru

Russian Federation

References

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