The experimental study of the preventive treatment with Viburnum sargentii K. and Eleutherococcus on the composition of fatty acids in phospholipids components of erythrocyte membranes exposed to intoxication with nitric oxides

Cover Page

Cite item

Full Text

Abstract

There are presented results of the study of the impact of intoxication with nitrogen oxides on to the fatty acids pattern of phosphatidylcholine and phosphatidylethanolamine of erythrocyte membrane in rats. The inhalation exposure with nitrogen oxides was carried out in inoculating chamber with the volume of 100 liters and designed according to the type of chambers by Kurliandsky B.A. with self-cleaning of contained air and regenerating system, specified temperature settings (20-22 °С) and the air humidity (40-60%). Intoxication by nitrogen oxides was performed for 6 minutes with a toxicant concentration of 4.3 mg/cu.m. (MAC in atmospheric air amount to 0.4 mg/m3). Water solutions of dry residue from viburnum extract “Kaliphen” and Eleutherococcus extract (alcohol was anticipatorily removed from preparations by evaporating in a vacuum) were administered daily through the tube intragastrically during 2 weeks in a dosage of 100 mg of total polyphenols per kg of body weight prior to intoxication by nitrogen oxides. The influence of nitrogen oxides was found to be accompanied by the elevation of the level of saturated fatty acids of all kinds and by the decline in unsaturated fatty acids content in the fatty acids pattern of phosphatidylcholine and phosphatidylethanolamine, the main structural phospholipids of biomembranes. The redistribution of fatty acids in erythrocyte membrane supposes the changes of its physicochemical properties, lability, and the complexity of the erythrocyte passage through the microvascular bed. Herbal polyphenol preparations Kaliphen and Eleutherococcus improved the survival rate of animals under the preventive administration before intoxication with nitrogen oxides. The introduction of extracts before intoxication is accompanied by the pronounced trend to the restoration of the ratio of saturated and unsaturated fatty acids in phospholipids of erythrocytes membranes. The preventive administration of Kaliphen before intoxication with nitrogen oxides was supposed to offer more pronounced protection of the fatty acids pattern of phospholipid fractions than those following the introduction of Eleutherococcus. The preventive administration of herbal extracts with polyphenol complexes in its contents under exposure to toxic substances is considered to be a the promising direction for research.

About the authors

Natalya F. Kushnerova

V.I. Ilichev Pacific Oceanological Institute

Author for correspondence.
Email: natasha50@mail.ru
ORCID iD: 0000-0002-6476-0039

MD, Ph.D., DSci., The head of the Department of Biochemical Technologies of the Subdivision: Laboratory of Biochemistry of the V.I. Ilichev Pacific Oceanological Institute, Vladivostok, 690041, Russian Federation.

e-mail: natasha50@mail.ru

Russian Federation

T. V. Momot

Far Eastern Federal University, School of biomedicine; National Scientific Center of Marine Biology

Email: noemail@neicon.ru
ORCID iD: 0000-0003-3873-0343
Russian Federation

Yu. A. Rakhmanin

Centre for Strategic Planning and Management of Biomedical Health Risks

Email: noemail@neicon.ru
ORCID iD: 0000-0003-2067-8014
Russian Federation

S. E. Fomenko

V.I. Ilichev Pacific Oceanological Institute

Email: noemail@neicon.ru
ORCID iD: 0000-0002-0261-0190
Russian Federation

V. G. Sprygin

V.I. Ilichev Pacific Oceanological Institute

Email: noemail@neicon.ru
ORCID iD: 0000-0001-7400-909X
Russian Federation

E. S. Drugova

V.I. Ilichev Pacific Oceanological Institute

Email: noemail@neicon.ru
ORCID iD: 0000-0002-7472-5958
Russian Federation

V. Yu. Merzliakov

V.I. Ilichev Pacific Oceanological Institute

Email: noemail@neicon.ru
ORCID iD: 0000-0002-9536-3247
Russian Federation

L. N. Lesnikova

V.I. Ilichev Pacific Oceanological Institute

Email: noemail@neicon.ru
ORCID iD: 0000-0003-4187-230X
Russian Federation

References

  1. Berchfild G., Storrs E. Gazovaya hromatografiya v biohimii. M.: Mir; 1964. (in Russian)
  2. Varlamov V.N. Chem nas «odarivaet» dizel’nyi gruzovik? Ekologicheskii vestnik Rossii. 2008; (2): 10-11. (in Russian)
  3. Vengerovskii A.N., Markova I.V., Saratikov A.S. Doklinicheskoe izuchenie gepatozashitnyh sredstv. Vedomosti farmakologicheskogo komiteta. 1999; (2): 9-12.
  4. Ivanova A.S., Pahrova O.A., Nazarov S.B. Vliyanie dlitel’noi nitritnoi intoksikacii na eritrocitarnuyu sistemu beremennyh krys i ih potomstvo. Gigiena i sanitariya. 2007; (2): 63-66. (in Russian)
  5. Ivanova A.S., Sitnikova O.G., Nazarov S.B. Sostoyanie svobodnoradikal’nyh processov u beremennyh krys i ih plodov pri hronicheskoi nitritnoi toksikacii. Gigiena i sanitariya. 2008; (4): 72-75. (in Russian)
  6. Il’nickii A.P. Nitraty i nitrity pit’evoi vody kak faktor onkologicheskogo riska. Gigiena i sanitariya. 2003; (3): 81-83. (in Russian)
  7. Kucenko S.A. Osnovy toksikologii. M.: Medicina; 2002. (in Russian)
  8. Kropotov A.V. Eksperimental’nyi otek legkih i ego farmakoprofilaktika antigipoksantami: avtoref. Dis….d-ra. med. nauk. S.-Pb.; 1997. (in Russian)
  9. Kushnerova N.F., Rahmanin Yu.A. Vliyanie intoksikacii oksidami azota na metabolicheskie reakcii pecheni i profilaktika porazhenii. Gigiena i sanitariya. 2008; (1): 70-73. (in Russian)
  10. Malysheva A.G., Sotnikov E.E., Moskovkin A.S. Nesimmetrichnyi dimetilgidrazin v okruzhayushei srede: problemy kontrolya i puti ih resheniya. Gigiena i sanitariya. 2003; (5): 74-75. (in Russian)
  11. Sidorov P.I., Skrebcova N.V., Sovershaeva S.L. Mediko-ekologicheskie aspekty zdorov’ya naseleniya na territoriyah raketno-kosmicheskoi deyatel’nosti. Gigiena i sanitariya. 2006; (3): 11-15. (in Russian)
  12. Sotnikova A.A., Sotnikova M.V., Dem’yanova V.S. Vliyanie zagryazneniya atmosfernogo vozduha na zabolevaemost’ naseleniya. Ekologiya i promyshlennost’ Rossii. 2006; (9): 44-45. (in Russian)
  13. Sprygin V.G., Kushnerova N.F. Kalina - novyi netradicionnyi istochnik oligomernyh proantocianidinov. Himiko-farmacevticheskii zhurnal. 2004; 38 (2): 41-45.
  14. Sprygin V.G., Kushnerova N.F., Fomenko S.E. Othody ot pererabotki dal’nevostochnyh dikorosov - perspektivnye istochniki pishevyh antioksidantov. Izvestiya Samarskogo nauchnogo centra RAN. 2010; 12 (1): 812-815. (in Russian)
  15. Fomenko S.E., Kushnerova N.F., Sprygin V.G., Momot T.V. Narushenie obmennyh processov v pecheni krys pod deistviem stressa. Tihookeanskii medicinskii zhurnal. 2013; (2): 67-70. (in Russian)
  16. Folch J., Less M., Sloane-Stanley G.H. A simple method for the isolation and purification of total lipids from animal tissue. Biol. Chem. 1957; 226: 497-509.
  17. Sija A., Scalese M., Lanza M. Flavonoids as antioxidant agents: importance of their interaction with biomembranes. Free Radic. Biol. Med. 1995; 19: 481-486.
  18. Sirk T.W., Friedman M., Brown E.F. Molecular binding of black tea theaflavins to biological membranes: relationship to bioactivities. J. Agric. Food Chem. 2011; 59: 3780-3787.
  19. Svetachev V.I., Vaskovsky V.E. A simplified technique for thin layer microchromatography of lipids. Chromatogr. 1972; 67 (2): 376-378.
  20. Valko M., Leibfritz D., Moncol J. Free radicals and antioxidant in normal physiological functions and human disease. The Int. J. of Biochem. and Cell Biol. 2007; 39: 44-84.

Supplementary files

Supplementary Files
Action
1. JATS XML

Copyright (c) 2024 Kushnerova N.F., Momot T.V., Rakhmanin Y.A., Fomenko S.E., Sprygin V.G., Drugova E.S., Merzliakov V.Y., Lesnikova L.N.



СМИ зарегистрировано Федеральной службой по надзору в сфере связи, информационных технологий и массовых коммуникаций (Роскомнадзор).
Регистрационный номер и дата принятия решения о регистрации СМИ: серия ПИ № ФС 77 - 37884 от 02.10.2009.