Chromatographic Determination of Ibuprofen in Rhodococcus Culture Media and Kinetic Modeling of Its Biodegradation Process

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Abstract

The conditions for the chromatographic determination of ibuprofen in culture media of Rhodococcus spp. by reversed-phase HPLC are chosen. The specificity, linearity, and accuracy of the developed procedure are confirmed by the validation procedure, which allows its use for a reliable assessment of ibuprofen concentration in the process of bacterial degradation. With the use of kinetic modeling, a forecast is made for changes in the concentration of ibuprofen; the half-life period, the end time of the biodegradation process, and its reproducibility are determined.

About the authors

E. V. Vikhareva

Perm National Research Polytechnic University; Perm State Pharmaceutical Academy

Email: vihareva@pfa.ru
614990, Perm, Russia; 614900, Perm, Russia

A. A. Selyaninov

Perm National Research Polytechnic University

Email: vihareva@pfa.ru
614990, Perm, Russia

G. A. Bazhutin

Perm Federal Research Center, Ural Branch, Russian Academy of Sciences

Email: vihareva@pfa.ru
614990, Perm, Russia

E. A. Tyumina

Perm Federal Research Center, Ural Branch, Russian Academy of Sciences

Author for correspondence.
Email: vihareva@pfa.ru
614990, Perm, Russia

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Copyright (c) 2023 Е.В. Вихарева, А.А. Селянинов, Г.А. Бажутин, Е.А. Тюмина