Functional Changes in the Expression of the Aqp4 Gene in the Hypothalamus Under the Influence of Drinking Regimen and Arterial Hypertension in Rats

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Abstract

Aquaporin-4 (AQP4) is the main water channel in the central nervous system. AQP4 is densely expressed in brain structures suggesting a crucial role in water transport in normal conditions and in disease. The effect of changes in drinking regime (water-deprivation and hyperhydration) and inherited arterial hypertension on the expression of the AQP4 water channel gene in the hypothalamus, the center of regulation of visceral functions, was studied. It was shown that the level of the Aqp4 mRNA in hyperhydrated animals more than 1.5 times lower than in animals with water-deprivation. This decrease in the Aqp4 gene expression in the hypothalamus may be associated with the ability to prevent cytotoxic edema during increased fluid intake. Rats with inherited stress induced arterial hypertension (ISIAH) are characterized by an increased level of Aqp4 mRNA in the hypothalamus, which suggests the involvement of this channel in processes associated with the regulation of brain water balance during arterial hypertension and the prevention of vasogenic cerebral edema. Thus, the presence of the AQP4 water channel in the brain, associated with the protection of brain cells, is functionally determined by the state of the body. This may be evidenced by bidirectional changes in the Aqp4 gene expression during hyperhydration and arterial hypertension.

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A. A. Evtushenko

Scientific Research Institute of Neurosciences and Medicine

Author for correspondence.
Email: evtushenkoaa@neuronm.ru
Russian Federation, Novosibirsk

I. V. Orlov

Scientific Research Institute of Neurosciences and Medicine; Novosibirsk State University

Email: evtushenkoaa@neuronm.ru
Russian Federation, Novosibirsk; Novosibirsk

I. P. Voronova

Scientific Research Institute of Neurosciences and Medicine

Email: evtushenkoaa@neuronm.ru
Russian Federation, Novosibirsk

T. V. Kozyreva

Scientific Research Institute of Neurosciences and Medicine; Novosibirsk State University

Email: evtushenkoaa@neuronm.ru
Russian Federation, Novosibirsk; Novosibirsk

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2. Fig. 1. The effect of drinking regimen (water deprivation and hyperhydration) on the expression of the Aqp4 gene in the posterior hypothalamus of rats. * – significant differences between the groups of water deprivation and hyperhydration, p < 0.05. The individual values are represented in the figure by triangles.

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3. Fig. 2. Expression of the Aqp4 gene in the anterior (white columns) and posterior (gray columns) hypothalamus of normal and hypertensive rats. * – significant differences between departments of the hypothalamus; # – significant differences between normal and hypertensive animals, p < 0.05. The individual values are represented in the figure by triangles.

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