EXPERIMENTAL STUDY OF THE HEMOSTATIC AND ANTIADHESIVE PROPERTIES OF MESOGEL IN THE CONDITIONS OF BLOOD AND BILE LEAKAGE IN LIVER INJURIES

Abstract

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Aim. Is to study in the experiment the hemostatic and anti-adhesive properties of Mesogel in conditions of blood and bile flow. Material and Methods. In vitro in two series of experiments in 55 studies, using the method of spectral analysis, the character of the change in the chemical properties of Mesogel in interaction with native blood and bile was studied. Under conditions of acute and chronic in vivo experiments, the time of bleeding stop and the amount of blood loss in a standard liver injury was studied in 90 rats, in control group and group using Mesogel. The anti-adhesive properties of the gel were studied on days 3, 7 and 14, by evaluating the adhesive process in the abdominal cavity using the method of the semantic differential. The results obtained were processed statistically. Results and Discussion. It is definitely revealed that Mesogel did not enter into chemical interaction with blood and bile. Preparation shortened the time of bleeding stop relative to the control at 109.19±34 seconds (by 37%; p≤0.05); reduced blood loss by 67±14 mg (by 15%, p≤0.05). In the chronic experiment the preservation of the anti-adhesive activity of the gel was confirmed: the absence of adhesive process on days 3, 7 and 14, the presence of only single viscero-parietal planar adhesions on day 14 in 12% of cases. Conclusions. In vitro studies have shown that when Mesogel is in contact with blood and bile, there is no chemical interaction and a change in its physical structure. In vivo studies have shown that Mesogel reliably has hemostatic and anti-adhesive activity. Mesogel can be used in the clinic for operations on the liver, as a combined action preparation.