THE EXPERIMENTAL EVALUATION OF THE TRANSGENERATIONAL EFFECT OF THE SYNESTROL ON THE MORPHOLOGY OF TESTICLES

Abstract

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Background. Prenatal exposure of various doses of estrogens and estrogen-like substances has a modulating effect on the morphogenesis of reproductive system organs, fertility and reproductive behavior of male offspring. However, the dose-dependent effects of this effect differ and the data from the various studies often contradict each other. Aim. The aim of this study was an experimental evaluation of postnatal structural and functional changes in the testicle parenchyma in the offspring of laboratory mice exposed during intrauterine period of the germinating of testes by the influence of injection of synestrol to their mothers. Material and Methods. The studies were carried out on pregnant females of laboratory mice weighing 23-25 g, divided into control and experimental groups. Pregnant females mice of the experimental group were administered by a subtoxic dose of 40 μg of synestrol per animal intramuscularly at stage E11.5. In the obtained male progeny, testicles were isolated on the 90th day of postnatal life and morphometric parameters of the spermatogenic epithelium were examined in them. Results and Discussion. As a result of the study, it was established that after the prenatal exposure of the synestrol, no significant differences in morphometric parameters were observed in the preparations of the testicles of experimental and control animals. There were no differences in the average area of cells of the spermatogenic epithelium, the number of cells per unit of the area, the total area of the spermatogenic epithelium, the area of the cytoplasm, and the nuclear-cytoplasmic ratio of the cells. Conclusions. Thus, high doses of synestrol during the testes germinating period in laboratory mice do not cause a transgenic prenatal effect on the structural and functional indices of spermatogenic epithelium in postnatal ontogenesis.