LYMPHOID STRUCTURES OF THE RAT SPLEEN UNDER EXPERIMENTAL CONDITIONS

Abstract

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Material and Methods. The microtopography of lymphoid structures of spleen of 212 Wistar male rats with different types of individual resistance to stress effects of experimental and control groups under emotional stress influence with preliminary injection of delta-sleep inducing peptide (DSIP) was studied. Results and Discussion. The percentage of lymphocytes in periarterial lymphoid sheath (PALS) in the spleen of rats, an hour after emotional stress (ES), decreased by an average of 1.2 times in comparison with the control groups animals. The content of lymphocytes after the injection of DSIP without stress in stable to stress rats increased to 62.3% or 14.5±0.2 cells (in control 50.9% or 13.6±0.1 cells) (p<0.05). In predisposed to stress rats after the injection of DSIP without stress, the content of this cells increased to 53.2% or 21.6±0.1 cells, (in control - 39.2% or 17.6±0.07 cells) (p<0.05). The lymphocyte content after one-hour stress influence with the preliminary injection of DSIP in resistant to stress rats increased insignificantly to 51.3% or 15.2±0.2 cells (in control 50.9% or 13.6±0.1 cells). Cells with destruction in the PALS of the spleen of control group (both predisposed and resistant to emotional stress) animals were found in isolated cases. Along with this, with the injection of DSIP, the content of macrophages and cells with destruction decreased in the experimental groups. Conclusions. Thus, it has been shown that the preliminary injection of DSIP increased the lymphocyte content, which inhibits the effect of emotional stress.