A NEW UNIVERSAL METHOD OF IMPREGNATING - NEW RESULTS IN MORPHOLOGY

Abstract

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Background. Methods of the impregnating of the nerve tissue and the walls of blood micro vessels which determined with solutions of silver salts, still remain empirical, little-informative, and give numerous artifacts. Aim. The aim of this study is to develop and test a universal method of selective detection of argyrophilic structures in various organs and tissues. Material and Methods. The investigation was carried out under etheric anesthesia on next experimental animals: on 5 inbred dogs, on inbred 7 cats and 17 white inbred laboratory rats. The first stage of the experiment was the preparatory procedure-perfusion into the bloodstream through the abdominal aorta of a 0.7% solution of salt of the silver nitrate AgNO3 and 0.1% hydroquinone (the authorship certificate of USSR № 1619, 08/09/1990). The second stage was an increasing of the argyrophility of the wall of the blood micro vessels and surrounding tissues with barium hydroxide. The third stage was the impregnation of frozen sections with a thickness of 25.0-100.0 μm and an square of up to 10.0- 15.0 cm2, which was modified by the histological method of Bielschowsky-Gross. Results and Discussion. The developed new method allows improving the quality and informativity of the impregnated preparations, the value and reliability of the results of histological studies. With using of this method new data have been obtained on the structural organization of the lymphatic vessels, and syncytial connections of neurocytes in the ganglia of the autonomous nervous system of the intestinal wall.