Email this article ЕXPRESSION OF DOUBLECORTIN AND NeuN IN THE DEVELOPING CEREBELLAR NEURONS IN RAT
- Authors: Zimatkin S.M.1, Karniushko O.A.1
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Affiliations:
- Grodno State Medical University
- Issue: Vol 149, No 1 (2016)
- Pages: 38-42
- Section: Articles
- URL: https://j-morphology.com/1026-3543/article/view/397624
- DOI: https://doi.org/10.17816/morph.397624
- ID: 397624
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Abstract
This work was performed on the offspring of 5 outbred female albino rats to give a comparative immunohistochemical evaluation of doublecortin (DCX) and NeuN expression in the neurons of the cerebellar cortex and nucleus interpositus in the early postnatal ontogenesis (postnatal days 2-15). DCX expression was detected in postmitotic neurons of the external granular layer and migrating neurons of the cerebellar cortex. At postnatal days 2 and 7 DCX expression in neocerebellum was higher than in paleocerebellum. NeuN expression was found to appear in migrating granule neurons, and reach the maximum in mature neurons of internal granular layer. DCX expression was not detected in Purkinje cells and in the nucleus interpositus of the cerebellum. In neurons of the nucleus interpositus the expression of NeuN progressively increased from postnatal days 2 to 15. Thus, a comparative immunohistochemical study of the dynamics of the expression of the pair of molecular markers studied proved to be an effective way of the assessment of the development of granular neurons of the cerebellum in early postnatal ontogenesis.
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About the authors
S. M. Zimatkin
Grodno State Medical University
Email: smzimatkin@grsmu.by
Department of Histology, Cytology and Embryology
O. A. Karniushko
Grodno State Medical University
Email: karnyushko-olga@mail.ru
Department of Histology, Cytology and Embryology
References
- Каркищенко Н. Н., Грачева С. В. Руководство по лабораторным животным и альтернативным моделям в биомедицинских исследованиях. М.: Профиль-2С, 2010.
- Коржевский Д. Э., Гилерович Е. Г., Зинькова Н. Н. и др. Иммуноцитохимическое выявление нейронов головного мозга с помощью селективного маркера NeuN // Морфология. 2005. Т. 128, вып. 5. С. 76-78.
- Коржевский Д. Э., Карпенко М. Н., Кирик О. В. Белки, ассоциированные с микротрубочками, как показатели дифференцировки функционального состояния нервных клеток // Морфология. 2011. Т. 139, вып. 1. С. 13-21.
- Коржевский Д. Э., Петрова Е. С., Кирик О. В. и др. Оценка дифференцировки нейронов в эмбриогенезе крысы с использованием иммуноцитохимического выявления даблкортина // Морфология. 2008. Т. 133, вып. 4. С. 7-10.
- Оленев С. Н. Развивающийся мозг. Л.: Наука, 1978.
- Altman J., Winfree A. T. Postnatal development of the cerebellar cortex in the rat. V. Spatial organization of Purkinje cell perikarya // J. Comp. Neurol. 1977. Vol. 171, № 1. P. 1-16.
- Friocourt G., Koulakoff A., Chafey P. et al. Doublecortin functions at the extremities of growing neuronal processes // Cereb. Cortex. 2003. Vol. 13, № 6. P. 620-626.
- Gleeson J. G., Lin P.T., Flanagan L. A. et al. Doublecortin is a microtubule-associated protein and is expressed widely by migrating neurons // Neuron. 1999. Vol. 23. P. 257-271.
- Hashimoto K., Kano M. Synapse elimination in the developing cerebellum // Cell. Mol. Life Sci. 2013. Vol. 70, № 24. P. 4667-4680.
- Kawaji K., Umeshima H., Eiraku M. et al. Dual phases of migration of cerebellar granule cells guided by axonal and dendritic leading processes // Mol. Cell. Neurosci. 2004. Vol. 25, № 2. P. 228-240.
- Komuro H., Yacubova E., Rakis P. Mode and tempo of tangential cell migration in the cerebellar external granular layer // J. Neurosci. 2001. Vol. 21, № 2. P. 527-540.
- Mullen R. J., Buck C. R., Smith A. M. NeuN, a neuronal specific nuclear protein in vertebrates // Development. 1992. Vol. 116, № 1. P. 201-211.
- Paolone N., Manohar S., HayesS. H. et al. Dissociation of doublecortin expression and neurogenesis in unipolar brush cells in the vestibulocerebellum and dorsal cochlear nucleus of the adult rat // Neuroscience. 2014. Vol. 265. P. 323-331.
- Rakis P., Knyihar-Csillik E., Csillik E. Polarity of microtubule assemblies during neuronal cell migration // Proc. Natl. Acad. Sci. USA. 1996. Vol. 93, № 17. P. 9218-9222.
- Takács J., Zaninetti R., Vig J. et al. Postnatal expression of Doublecortin (DСХ) in the developing cerebellar cortex of mouse // Acta Biol. 2008. Vol. 59, № 2. P. 147-161.
- Wingate R. J. The rhombic lip and early cerebellar development // Curr. Opin. Neurobiol. 2001. Vol. 11, № 1. P. 82-88.
- Yacubova E., Komuro H. Intrinsic program for migration of cerebellar granule cells in vitro // J. Neurosci. 2002. Vol. 22, № 14. P. 5966-5981.
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СМИ зарегистрировано Федеральной службой по надзору в сфере связи, информационных технологий и массовых коммуникаций (Роскомнадзор).