CURRENT HISTOCHEMICAL METHODS OF TISSUE IRON DEMONSTRATION BASED ON PERLS’ REACTION



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Abstract

The article presents the information on the modern modifications of Perls’ reaction including diaminobenzidine enhancement according to R. Meguro (for various tissues), M. A. Smith (for nervous tissue), S. M. Levine (for detection of iron in oligodendrocytes and myelinated nervous fibers), and our own modification for visualization of the nucleolar iron. The analysis is performed of the advantages and disadvantages of these modifications of histochemical demonstration of iron. It is shown that the use of new methodological approaches significantly increases the sensitivity of Perls’ reaction. Control procedures allow to to eliminate the possibility of artifacts.

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About the authors

I. P. Grigoriyev

RAS Research Institute of Experimental Medicine

Email: ipg-iem@yandex.ru
Laboratory of Functional Morphology of the Central and Peripheral Nervous System, Department of General and Special Morphology

Ye. A. Kolos

RAS Research Institute of Experimental Medicine

Laboratory of Functional Morphology of the Central and Peripheral Nervous System, Department of General and Special Morphology

Ye. G. Sukhorukova

RAS Research Institute of Experimental Medicine

Email: len48@inbox.ru
Laboratory of Functional Morphology of the Central and Peripheral Nervous System, Department of General and Special Morphology

D. E. Korzhevskiy

RAS Research Institute of Experimental Medicine

Email: iemmorphol@yandex.ru
Laboratory of Functional Morphology of the Central and Peripheral Nervous System, Department of General and Special Morphology

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