Email this article SPERM MORPHOLOGICAL CHARACTERISTICS IN YOUNG MEN: ASSOCIATION WITH MOTILITY
- Authors: Osadchuk L.V.1, Kleshchev M.A.1, Kuznetsova N.N.2, Osadchuk A.V.1
-
Affiliations:
- Federal Research Center Institute of Cytology and Genetics
- «Ergin» Medical Center
- Issue: Vol 151, No 1 (2017)
- Pages: 49-56
- Section: Articles
- URL: https://j-morphology.com/1026-3543/article/view/397800
- DOI: https://doi.org/10.17816/morph.397800
- ID: 397800
Cite item
Open Access
Access granted
Abstract
Male fertility is associated with the characteristics of spermatogenesis, particularly with sperm morphology. The aim of this study was to analyze morphological characteristics of sperm cells in relation to their motility, as well as to compare incidence of morphological sperm defects in young men with normal and impaired spermatogenesis. Morphological characteristics of sperm cells were analyzed in young men (n = 111, age: 21.0±0.2 years) according to WHO «strict criteria» (2010). Among morphological abnormalities of sperm cells, the head defects (amorphous or vacuolated head) and acrosome and midpiece abnormalities predominated. In the group of men with reduced sperm motility (asthenozoospermia), the proportion of sperm cells with abnormal acrosome was higher than in norm. In the group of men with reduced sperm concentration, reduced proportion of motile and morphologically normal sperm cells (asthenooligoteratozoospermia), the increased percentage of sperm cells with abnormal acrosome, vacuolated and bent heads, thickened midpiece, cytoplasmic droplet, coiled and short tail was observed. In the group of men with normal sperm parameters, there was a negative correlation between the incidence of acrosome abnormalities and the percentage of motile sperm cells, while in the group of men with asthenozoospermia no correlation was found between the incidence of any morphological defects and the percentage of motile sperm cells. In the group of men with asthenooligoteratozoospermia, negative correlation was detected between the percentage of motile sperm cells and the incidence of of bent heads, coiled and short tails. The data obtained indicate a connection between morphological parameters and sperm motility. Increased incidence of acrosome morphological abnormalities may serve as a morphological marker of impaired spermatogenesis.
Full Text
About the authors
L. V. Osadchuk
Federal Research Center Institute of Cytology and Genetics
Email: losadch@bionet.nsc.ru
M. A. Kleshchev
Federal Research Center Institute of Cytology and Genetics
Email: max82cll@bionet.nsc.ru
N. N. Kuznetsova
«Ergin» Medical Center
Email: erginmc@gmail.com
A. V. Osadchuk
Federal Research Center Institute of Cytology and Genetics
Email: osadchuk@bionet.nsc.ru
References
- Андрология: мужское здоровье и дисфункция репродуктивной системы / Под ред. Э. Нишлага, Г. М. Бере. М.: ООО «Медицинское информационное агентство», 2005.
- Осадчук Л. В., Тупикин А. Е., Морозов И. В. и др. Фенотипическая вариабельность сперматогенеза и поиск ассоциаций с генным полиморфизмом у мышей 13 инбредных линий // Генетика. 2012. № 8. С. 966-975.
- Попова А. В., Клещев М. А., Осадчук А. В. и др. Морфологический анализ сперматозоидов и связь их аномалий с показателями спермограммы // Вестн. НГУ. Сер. «Биология. Клин. медицина». 2011. Т. 9, № 3. С. 47-54.
- Abu Hassan Abu D., Franken D. R., Hoffman B., Henkel R. Accu rate sperm morphology assessment predicts sperm function // Andrologia. 2012. Vol. 44. P. 571-577.
- Agarwal A., Roychoudhury S., Bjugstad K. B. et al. Oxidation-reduction potential of semen: what is its role in the treatment of male infertility? // Ther. Adv. Urol. 2016. Vol. 8, № 5. P. 302-318.
- Auger F., Eustache G., Andersen A. G. et al. Sperm morphological defects related to environment, lifestyle and medical history of 1001 male partners of pregnant women from four European cities // Hum. Reprod. 2001. Vol. 16. P. 2710-2717.
- Chemes H. E., Rawe V. Y. Sperm pathology: a step beyond descriptive morphology. Origin, characterization and fertility potential of abnormal sperm phenotypes in infertile men // Hum. Reprod. Update. 2003. Vol. 9. P. 405-428.
- Cooper T. G., Noonan E., Eckardstein S. et al. World Health Organization reference values for human semen characteristics // Hum. Reprod. Update. 2010. Vol. 16. P. 231-245.
- De Braekeleer M., Nguyen M. H., Morel F. et al. Genetic aspects of monomorphic teratozoospermia: a review // J. Assist. Reprod. Genet. 2015. Vol. 32. P. 615-623.
- Elshal M. F., El-Sayed I. H., Elsaied M. A. et al. Sperm head defects and disturbances in spermatozoal chromatin and DNA integrities in idiopathic infertile subjects: Association with cigarette smoking // Clin. Biochem. 2009. Vol. 42. P. 589-594.
- Fischer M. A., Willis J., Zini A. Human sperm DNA integrity: correlation with sperm cytoplasmic droplets // Urology. 2003. Vol. 61. P. 207-211.
- Harbuz R., Zouari R., Pierre V. A. Recurrent deletion of DPY19L2 causes infertility in man by blocking sperm head elongation and acrosome formation // Amer. J. Hum. Gen. 2011. Vol. 88. P. 351-361.
- Jensen T. K., Swan S., Jørgensen N. et al. Alcohol and male reproductive health: a cross-sectional study of 8344 healthy men from Europe and the USA // Hum. Reprod. 2014. Vol. 29, № 8. P. 1801-1809.
- Jørgensen N., Carlsen E., Nermoen I. et al. East-West gradient in semen quality in the Nordic-Baltic area: a study of men from the general population in Denmark, Norway, Estonia and Finland // Hum. Reprod. 2002. Vol. 17. P. 2199-2208.
- Khosrowbeygi A., Zarghami N. Seminal plasma levels of free 8-isoprostane and its relationship with sperm quality parameters // Indian J. Clin. Biochem. 2008. Vol. 23, № 1. P. 49-52.
- La Vignera S., Condorelli R. A., Vicari E. et al. Negative effect of increased body weight on sperm conventional and nonconventional flow cytometric sperm parameters // J. Androl. 2012. Vol. 33. P. 53-58.
- Menkveld R. Clinical significance of the low normal sperm morphology value as proposed in the fifth edition of the WHO Laboratory Manual for the examination and processing of human semen // Asian J. Androl. 2010. Vol. 12. P. 47-58.
- Menkveld R., Holleboom C. A. G., Rhemrev J. R. T.Measurement and significance of sperm morphology // Asian J. Androl. 2011. Vol. 13. P. 59-68.
- Menkveld R., Kruger T. F. Sperm morphology and male urogenital infections // Andrologia. 1998. Vol. 30 (Suppl. 1). P. 49-53.
- Nordkap L., Jensen T. K., Hansen A. M. et al. Psychological stress and testicular function: a cross-sectional study of 1,215 Danish men // Fert. Steril. 2016. Vol. 105. P. 174-187.
- Parinaud J., Vieitez G., Moutaffian H. et al. Relationships between motility parameters, morphology and acrosomal status of human spermatozoa // Hum. Reprod. 1996. Vol. 11. P. 1240-1243.
- Piasecka M., Laszczyńska M., Gączarzewicz D. Morphological and functional evaluation of spermatozoa from patients with asthenoteratozoospermia // Folia Morphol. (Warsz.). 2003. Vol. 62. P. 479-481.
- Portuondo J. A., Calabozo M., Echanojauregui A. D. Morphology of spermatozoa in infertile men with and without varicocele // J. Androl. 1983. Vol. 4. P. 312-315.
- WHO Laboratory Manual for the Examination and Processing of Human Semen, 5th ed. Geneva, Switzerland: WHO Press, 2010.
Supplementary files
СМИ зарегистрировано Федеральной службой по надзору в сфере связи, информационных технологий и массовых коммуникаций (Роскомнадзор).