TYPE I COLLAGEN, SIRTUIN-6, AND MATRIX METALLOPROTEINASE-1 EXPRESSION IN HUMAN SKIN FIBROBLASTS DURING LONG-TERM CULTIVATION



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Abstract

Objective - to verify the senescence markers of skin fibroblasts in in vitro model. Material and methods. The study was performed on cultured human skin fibroblasts of 3rd passage (“young» cultures) and 14th passage (“old” cultures). The expression of type I collagen, sirtuin-6 and matrix mettaloproteinase-1 (MMP-1) in skin fibroblasts cultures was examined using the method of immunofluorescence confocal microscopy. Results . It was shown that during cell aging, the expression of type I collagen and sirtuin-6 in skin fibroblasts cultures decreased 3.5 and 3.6 times respectively, while the expression of MMP-1 increased 2.5 times. Conclusions . It is suggested, that decreasing sirtuinn-6 expression results in the disturbances of type I collagen synthesis and increase in MMP-1 expression. This mechanism can play an important role in the understanding of molecular mechanisms of skin aging.

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About the authors

N. V. Fridman

St. Petersburg Bioregulation and Gerontology Institute

Email: ibg@gerontology.ru
Department of Biogerontology, Department of Cell Biology and Pathology

N. S. Lin’kova

St. Petersburg Bioregulation and Gerontology Institute; Peter the Great St. Petersburg Polytechnic University

Email: miayy@yandex.ru
Department of Biogerontology, Department of Cell Biology and Pathology; Department of Medical Physics; Institute of Physics, Nanotechnology and Telecommunications

V. O. Polyakova

D. O. Ott Scientific-Research Institute of Obstetrics, Gynecology and Reproduction; St. Petersburg State University

Email: vopol@yandex.ru
Department of Pathomorphology; Departments of Pathology and Physiology

A. O. Drobintseva

D. O. Ott Scientific-Research Institute of Obstetrics, Gynecology and Reproduction; St Petersburg State Pediatric Medical University

Email: anna-flor@mail.ru
Department of Pathomorphology; Department of Medical Biology

S. V. Trofimova

St. Petersburg Bioregulation and Gerontology Institute

Email: dr.s.trofimova@gmail.com
Department of Biogerontology, Department of Cell Biology and Pathology

A. V. Dudkov

St. Petersburg Bioregulation and Gerontology Institute

Department of Biogerontology

V. Kh. Khavinson

St. Petersburg Bioregulation and Gerontology Institute; RAS I. P. Pavlov Institute of Physiology

Department of Biogerontology; Group of Peptide Regulation of Ageing

I. M. Kvetnoy

St. Petersburg Bioregulation and Gerontology Institute; D. O. Ott Scientific-Research Institute of Obstetrics, Gynecology and Reproduction; St. Petersburg State University

Department of Biogerontology; Department of Pathomorphology; Departments of Pathology and Physiology

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