MORPHOLOGICAL RESTRUCTURING OF KIDNEYS IN RATS WITH ALLOXAN DIABETES SUBJECTED TO STIMULATION OF MACROPHAGE FUNCTIONAL ACTIVITY



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Abstract

Objective. To assess the structure of the kidney in rats with alloxan diabetes mellitus (DM) following modulation of the macrophage functional activity. Material and methods. In 42 Wistar rats with alloxan DM (a dose of alloxan 300 mg/kg) with average weight 210 g, macrophage activity was modulated by administration of aminophthalhydrazide (2 mg/kg). In histological sections, the morphometric parameters of nephrons were studied, and the expression of Ki-67 in cells and of CD68 in macrophages was examined with immunohistochemical staining. The blood levels of glucose, glycated hemoglobin, insulin, urea, and creatinine were determined. Results. Modulation of macrophage activity in rats with diabetic nephropathy promoted an increase in the proliferative activity of the glomerular сell and tubular epithelial cells, a decrease in the percentage of damaged glomeruli. Conclusions. Modulation of the functional activity of macrophages can reduce the level of structural damage in the kidneys caused by diabetes in rats.

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About the authors

I. G. Danilova

First President of Russia B. N. Yeltsin Ural Federal University; RAS Ural Branch, Institute of Immunology and Physiology

Email: ig-danilova@yandex.ru
Department of Medical Biochemistry and Biophysics; Laboratory of Morphology and Biochemistry 19 Mira Str., Yekaterinburg 620002; 106 Pervomaiskaya Str., Yekaterinburg 620049

V. A. Chereshnev

RAS Ural Branch, Institute of Immunology and Physiology

Email: secretar@iip.uran.ru
Laboratory of Immunophysiology and Immunopharmocology 106 Pervomaiskaya Str., Yekaterinburg 620049

N. B. Blinkova

RAS Ural Branch, Institute of Immunology and Physiology

Email: n.b.krohina@mail.ru
Laboratory of Morphology and Biochemistry 106 Pervomaiskaya Str., Yekaterinburg 620049

S. Yu. Medvedeva

First President of Russia B. N. Yeltsin Ural Federal University; RAS Ural Branch, Institute of Immunology and Physiology

Email: medvedeva-ran@yandex.ru
Department of Medical Biochemistry and Biophysics; Laboratory of Morphology and Biochemistry 19 Mira Str., Yekaterinburg 620002; 106 Pervomaiskaya Str., Yekaterinburg 620049

I. F. Gette

First President of Russia B. N. Yeltsin Ural Federal University; RAS Ural Branch, Institute of Immunology and Physiology

Email: i.goette@yandex.ru
Department of Immunochemistry; Laboratory of Morphology and Biochemistry 19 Mira Str., Yekaterinburg 620002; 106 Pervomaiskaya Str., Yekaterinburg 620049

A. V. Belousova

First President of Russia B. N. Yeltsin Ural Federal University; RAS Ural Branch, Institute of Immunology and Physiology

Email: a1b2v3@mail.ru
Department of Immunochemistry; Laboratory of Morphology and Biochemistry 19 Mira Str., Yekaterinburg 620002; 106 Pervomaiskaya Str., Yekaterinburg 620049

M. T. Abidov

First President of Russia B. N. Yeltsin Ural Federal University

Email: alina44@mail.ru
Department of Medical Biochemistry and Biophysics 19 Mira Str., Yekaterinburg 620002

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