THE DISTRIBUTION OF NADPH-DIAPHORASE AND NEURONAL NO SYNTHASE IN RAT MEDULLA OBLONGATA NUCLEI
- Authors: Chertok V.M1, Kotsyuba A.Y.1
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Affiliations:
- Vladivostok State Medical University
- Issue: Vol 144, No 6 (2013)
- Pages: 009-014
- Section: Articles
- URL: https://j-morphology.com/1026-3543/article/view/398675
- DOI: https://doi.org/10.17816/morph.398675
- ID: 398675
Cite item
Abstract
The distribution of nitroxide ergic neurons in the medulla oblongata nuclei in Wistar rats (n=8) was studied histochemically (NADPH-diaphorase) and using immunohistochemistry with an antiserum against neuronal form of nitric oxide synthase (nNOS). NADPH-diaphorase activity was found in large and small neurons of the sensory, autonomic and motor nuclei. The latter were especially rich in the cells demonstrating the activity of the enzyme. Unlike NADPH-diaphorase, nNOS in the corresponding nuclei was always detected in the fewer number of neurons, predominantly of small sizes. The sensory nuclei (nucleus of solitary tract, reticular parvocellular and lateral nuclei, spinal nucleus of the trigeminal nerve) contained 1.5–3 times more nNOS neurons than in motor nuclei. In some nuclei (nucleus ambiguus, hypoglossal nerve nucleus), containing numerous NADPH-diaphorase-positive neurons, immunoreactive cells were particularly rare
Full Text
About the authors
V. M Chertok
Vladivostok State Medical University
Email: chertoky@mail.ru
A. Ye. Kotsyuba
Vladivostok State Medical University
Email: akotc@mail.ru
References
- Афанасьев А. А., Коцюба А. Е. и Черток В. М. Система для автоматизированного анализа изображений микро-и макроструктур Allegro-MC. Тихоокеанский мед. журн., 2002, № 4, с. 65–68.
- Коцюба А. Е., Коцюба Е. П. и Черток В. М. Нитроксидергические нервные волокна внутримозговых сосудов. Морфология, 2009, т. 135, вып. 2, с. 27–32.
- Порсева В. В. и Шилкин В. В. NADPH-диафоразопозитивные структуры спинного мозга и спинномозговых узлов. Морфология, 2010, т. 137, вып. 2, с. 13–17.
- Старцева М. С., Шуматова Т. А., Маркина Л. Д. и Мотавкин П. А. Сравнительная характеристика NADPH-позитивных нейронов головного мозга человека и крысы. Тихоокеанский мед. журн., 2012, № 3, с. 74–76.
- Черток В. М. и Коцюба А. Е. NO-позитивные нейроны в ядрах бульбарного вазомоторного центра человека при артериальной гипертензии. Бюл. экспер. биол., 2009, т. 147, № 5, с. 571–575.
- Черток В. М. и Коцюба А. Е. Оксид азота в механизмах афферентной иннервации артерий головного мозга. Цитология, 2010, № 1, с. 24–29.
- Черток В. М., Коцюба А. Е. и Бабич Е. В. Эфферентная иннервация артерий мягкой оболочки мозга человека при артериальной гипертензии. Морфология, 2009, т. 135, вып. 3, с. 35–41.
- Abudara V., Ferna A., Chase M. H. and Morales F. Nitric Oxide as an Anterograde Neurotransmitter in the Trigeminal Motor Pool. J. Neurophysiol., 2002, v. 88, p. 497–506.
- Atkinson L., Batten T. F., Corbett E. K. et al. Subcellular localization of neuronal nitric oxide synthase in the rat nucleus of the solitary tract in relation to vagal afferent inputs. Neuroscience, 2003, v. 118, № 1, p. 115–122.
- Brüning G., Katzbach R. and Mayer B. Histochemical and immunocytochemical localization of nitric oxide synthase in the central nervous system of the goldfish, carassiusauratus. J. Comp. Neurol., 1995, v. 358, № 3, p. 353–382.
- Dawson T. M., Bredt D. S., Fotuhi M. et al. Nitric oxide synthase and neuronal NADPH diaphorase are identical in brain and peripheral tissues. Proc. Natl. Acad. Sci. USA, 1991, v. 88, p. 7797–7801.
- Esplugues J. V. NO as a signalling molecule in the nervous system. British J. Pharmacol., 2002, v. 135, № 5, p. 1079–1095.
- Giraldez-Perez R. M., Gaytan S. P., Ruano D. et al. Distribution of NADPH-diaphorase and nitric oxide synthase reactivity in the central nervous system of the goldfish (Carassiusa uratus). J. Chem. Neuroanat., 2008, v. 35, № 1, p. 12–32.
- González-Hernandez T., Perez de la Cruz M. A. and Mantolan-Sarmiento B. Histochemical and immunohistochemical detection of neurons that produce nitric oxide: effect of different fixative parameters and immunoreactivity against non-neuronal NOS antisera. J. Histochem., Cytochem., 1996, v. 44, p. 1399–1413.
- Hope B. T., Michael G. J., Knigge K. M. and Vincent S. R. Neuronal NADPH diaphorase is a nitric oxide synthase. Proc. Natl. Acad. Sci. USA, 1991, v. 88, p. 2811–2814.
- Hope B. T. and Vincent S. R. Histochemical characterization of neuronal NADPH-diaphorase. J. Histochem. Cytochem., 1989, v. 37, p. 653–661.
- Huynh P. and Boyd S. K. Nitric oxide synthase and NADPH-diaphorase distribution in the Bullfrog (Rana catesbeiana) CNS: pathways and functional implications. Brain Behav. Evol., 2007, v. 70, p. 145–163.
- Thomas E. and Pearse A. G.E. The solitary active cells: histochemical demonstration of damage-resistant nerve cells with TPN-diaphorase reaction. Acta Neuropathol., 1964, v. 3, p. 238–249.
- Traub R. J., Solodkin A., Meller S. T. and Gebhart G. F. Spinal cord NADPH-diaphorase histochemical staining but not nitric oxide synthase immunoreactivity increases following carrageen in produced hind paw in ammation in the rat. Brain Res., 1994, v. 668, p. 204–210.
- Tseng C. Y., Lue J. H., Chang H. M. et al. Ultrastructura llocalisation of NADPH-d/nNOS expression in the superior cervical ganglion of the hamster. J. Anat., 2000, v. 197, № 3, p. 461–75.
- Wolf G. Nitric oxide and nitric oxide synthase: biology, pathology, localization. Histol. Histopathol., 1997, v. 12, р. 251–261.