REACTIONS OF THE INTERNEURONAL SYNAPSES OF RAT BRAIN TO HYPOXIA DURING THE EARLY POSTNATAL PERIOD
- Authors: Otellin V.A1, Khozhai L.I.1, Shishko T.T.1
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Affiliations:
- RAS I. P.Pavlov Institute of Physiology
- Issue: Vol 145, No 1 (2014)
- Pages: 7-12
- Section: Articles
- URL: https://j-morphology.com/1026-3543/article/view/398714
- DOI: https://doi.org/10.17816/morph.398714
- ID: 398714
Cite item
Abstract
The reactions of forming synapses in rat neocortex to the effect of hypoxia in the early postnatal period (day 2) were studied. Using immunocytochemistry for synaptophysin demonstratoion and electron microscopic methods, the sensorimotor cortex was studied in rats at days 3, 4 and 10 of postnatal development (6 to 10 animals of each age) in both experimental and control groups (intact animals). Immunocytochemical study of the control animals demonstrated significant differences in the quantitative distribution of synaptophysin-positive structures in the different layers of the neocortex in the early postnatal period of development (day 5). It is shown that after exposure to perinatal hypoxia, more than 2-fold decrease of the optical density of the immunocytochemical reaction product took place together with the reduction of synaptophysin-positive granules distribution density in all cortical layers of. At the same time, electron-dense terminals demonstrating early degenerative processes were found. In the neuropil of the neocortex, a sharp decline in the number of growth cones, small processes and forming synapses was accompanied by significant changes of the electron density of synaptic, especially post-synaptic, membranes and densities. In the experimental animals, the number of growth cones and emerging synaptic structures were shown to increase only by postnatal day 10. Thus, the effects of hypoxia in the early postnatal period, causing disturbances of synaptogenesis, persist throughout the whole neonatal period examined.
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About the authors
V. A Otellin
RAS I. P.Pavlov Institute of Physiology
Email: votellin@mail.ru
L. I. Khozhai
RAS I. P.Pavlov Institute of Physiology
Email: astarta0505@mail.ru
T. T. Shishko
RAS I. P.Pavlov Institute of Physiology
References
- Лебедев С. В., Карасев А. В., Рогаткин С. О. и др. Проблемы и перспективы экспериментального моделирования гипоксически-ишемических повреждений центральной нервной системы. Вестн. Рос. акад. мед. наук, 2009, № 2, c. 21–26.
- Ушаков И. Б. и Федоров В. П. Кислород. Радиация. Мозг. Воронеж, Научная книга, 2011.
- Cebral E. and Loidl C. F. Changes in neostriatal and hippocampal synaptic densities in perinatal asphyctic male and female young rat: role of hypothermia. Brain Res. Bull., 2011, v. 84, № 1, p. 31–38.
- Clancy В., Darlington R. В. and Finlay B. L. Translating developmental time across mammalian species. Neuroscience, 2001, v. 105, № 1, p. 7–17.
- Dean J. M., Moravec M. D., Grafe M. et al. Strain-specific differences in perinatal rodent oligodendrocyte lineage progression and its correlation with human. Dev. Neurosci., 2011, v. 33, № 3–4, p. 251–260.
- Garcia-Penas J. J., Dominguez-Carral J. and Pereira-Bezanilla E. Abnormalities of synaptogenesis in autism. Pathogenic and therapeutic implications. Rev. Neurol., 2012, v. 54, Suppl. 1, p. 1–50.
- Laeme L. K., Repke K. B., Hawkes R. and Rice F. L. Synaptogenesis in the rat suprachiasmatic nucleus: a light microscopic immu nocytochemical survey. Brain Res., 1991, v. 544, № 1, p. 108–117.
- Li M., Cui Z., Niu Y. et al. Synaptogenesis in the deve loping mouse visual cortex. Brain Res. Bull., 2010, v. 81, № 1, p. 107–113.
- Pyeon H. J. and Lee Y. I. Differential expression levels of synaptophysin through developmental stages in hippocampal region of mouse brain. Anat. Cell Biol., 2012, v. 45, № 2, p. 97–102.
- Saraceno G. E., Avala M. V., Badorrey M. S. et al. Effects of perinatal asphyxia on rat striatal cytoskeleton. Synapse, 2012, v. 66, № 1, p. 9–19.
- Sarnat H. B., Auer R. N. and Flores-Sarnat L. Synaptogenesis in the fetal corpus striatum, globus pallidus, and substantia nigra: correlation with striosomes of Graybiel and dyskinesias in premature infants. J. Child Neurol., 2013, v. 28, № 1, p. 60–69.
- Sarnat H. B. and Born D. E. Synaptophysin immunocytochemistry with thermal intensification: a marker of terminal axonal maturation in the human fetal nervous system. Brain Dev., 1999, v. 21, № 1, p. 41–50.
- Sarnat H. B., Flores-Sarnat L. and Trevenen C. L. Synaptophysin immunoreactivity in the human hippocampus and neocortex from 6 to 41 weeks of gestation. J. Neuropathol. Exp. Neurol., 2010, v. 69, № 3, p. 234–245.
- Strackx E., Van den Hove D. L., Steinbusch H. P. et al. Fetal asphyxia leads to the loss of striatal presynartic boutons in adult rats. Int. J. Dev. Neurosci., 2010, v. 28, № 3, p. 277–281.
- Vannucci S. J. and Hagberg H. Hypoxia-ishemia in the immature brain. J. Exp. Biol., 2004, v. 207, № 18, p. 3149–3154.