CALBINDIN-IMMUNOREACTIVE INTERNEURONS OF THE SPINAL CORD GRAY MATTER INTERMEDIATE ZONE AND VENTRAL HORNS IN ALBINO RAT



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Abstract

The aim of the study was to examine the topography and structural characteristics of the interneurons containing 28 kDa calbindin (CAB) in the ventral horn and the intermediate zone of the spinal cord (SC) gray matter in T II and L IV segments. The study was performed on 4 adult female Wistar rats using immunohistochemical and morphometric methods. 14 micrometers-thick cryostat cross- sections of SC were analyzed. Two subpopulations of CAB-immunoreactive (CAB-IR) interneurons were present in the intermediate SM zone in lamina VII in both segments: preganglionic sympathetic neurons and the partition cells. In the ventral horn, also two subpopulations of CAB-IR interneurons were identified: Renshaw cells in the lamina IX in T II and in the lamina VII in L IV; large interneurons in the lamina VIII in and lamina VII in L IV. Segmental differences were observed TIIonly in larger numbers of Renshaw cells and partition cells in L IV. The average cross-sectional area of CAB-IR Renshaw cells and autonomic preganglionic neurons was greater in T II, while this parameter of large ventral horn interneurons and partition cells was greater in L IV.

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About the authors

V. V. Porseva

Yaroslavl’ State Medical Academy

Email: vvporseva@mail.ru

References

  1. Маслюков П. М., Коробкин А. А., Коновалов В. В. и др. Возрастное развитие кальбиндин-иммунопозитивных нейронов симпатических узлов крысы. Морфология, 2012, т. 141, вып. 1, с. 77-80.
  2. Alvarez F. J., Benito-Gonzalez A. and Siembab V. C. Principles of interneuron development learned from Renshaw cells and the motoneuron recurrent inhibitory circuit. Ann. N Y Acad. Sci., 2013, v. 1279, p. 22-31.
  3. Alvarez F. J. and Fyffe R. E. W. The continuing case for the Renshaw cell. J. Physiol., 2007, v. 584, p. 31-45.
  4. Anelli R. and Heckman C. J. The calcium binding proteins calbindin, parvalbumin, and calretinin have specific patterns of expression in the gray matter of cat spinal cord. J. Neurocytol., 2006, v. 34, № 6, р. 369-385.
  5. Barber R. P., Phelps P. E., Houser C. R. et al. The morphology and distribution of neurons containing choline acetyltransferase in the adult rat spinal cord: an immunocytochemical study. J. Comp. Neurol., 1984, v. 229, p. 329-346.
  6. Benito-Gonzalez A. and Alvarez F. J. Renshaw cells and Ia inhibitory interneurons are generated at different times from p1 progenitors and differentiate shortly after exiting the cell cycle. J. Neurosci., 2012, v. 32, № 4, р. 1156-1170.
  7. Clarke H. A., Dekaban G. A. and Weaver L. C. Identification of lamina V and VII interneurons presynaptic to adrenal sympathetic preganglionic neurons in rats using a recombinant herpes simplex virus type 1. J. Neurosci., 1998, v. 85, № 3, p. 863-872.
  8. Deuchars S. A., Milligan C. J., Stornetta R. L. and Deuchars J. GABAergic neurons in the central region of the spinal cord: a novel substrate for sympathetic inhibition. J. Neurosci., 2005, v. 25, № 5, р. 1063-1070.
  9. Goulding M. Circuits controlling vertebrate locomotion: moving in a new direction. Nat. Rev. Neurosci., 2009, v. 10, № 7, p. 507-518.
  10. Jankowska E. Spinal interneuronal networks in the cat: elementary components. Brain Res. Rev., 2008, v. 57, p. 46-55.
  11. Jankowska E., Bannatyne B. A., Liu T.T. et al. Commissural interneurones with input from muscle afferents in midlumbar segments in the cat; axonal projections, transmitter content and target cells. J. Physiol., 2009, v. 587, p. 401-418.
  12. Llewellyn-Smith I. J., Martin C. L. and Minson J. B. Glutamate and GABA content of calbindin-immunoreactive nerve terminals in the rat intermediolateral cell column. Auton. Neurosci., 2002, v. 98, № 1-2, р. 7-11.
  13. Lunam C. A. Calbindin immunoreactivity in the neurons of the spinal cord and dorsal root ganglion of the domestic fowl. Cell Tissue Res., 1989, v. 257, № 1, р. 149-153.
  14. Puskбr Z. and Antal M. Localization of last-order premotor interneurons in the lumbar spinal cord of rats. J. Comp. Neurol., 1997, v. 389, № 3, р. 377-89.
  15. Rexed B. The cytoarchitectonic organization of the spinal cord of the cat. J. Сomp. Neurol., 1952, v. 96, p. 415-495.
  16. Saywell S. A., Ford T.W., Meehan C. F. et al. Electrophysiological and morphological characterization of propriospinal interneurons in the thoracic spinal cord. J. Neurophysiol, 2011, v. 105, p. 806-826.
  17. Steiner T. J. and Turner L. M. Cytoarchitecture of the rat spinal cord. J. Physiol., 1972, v. 222, p. 123-125.
  18. Stepien A. E., Tripodi М. and Arber S. Monosynaptic rabies virus reveals premotor network organization and synaptic specificity of cholinergic partition cells. Neuron, 2010, v. 68, p. 456-472.

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