HISTOPHYSIOLOGY OF THE TRACHEAL EPITHELIUM IN RATS IN POSTNATAL ONTOGENESIS
- Authors: Pavlov A.V.1, Yesev L.I.1
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Affiliations:
- Yaroslavl’ State Medical Academy
- Issue: Vol 146, No 6 (2014)
- Pages: 80-86
- Section: Articles
- URL: https://j-morphology.com/1026-3543/article/view/398842
- DOI: https://doi.org/10.17816/morph.398842
- ID: 398842
Cite item
Abstract
Using a complex methodology of in vivo monitoring of the motor activity of the ciliary apparatus in combination with histological and morphometric methods, the epithelial lining of the trachea 110 Wistar rats was studied from birth until old age (24 months). In newborn animals, tracheal epithelium was characterized by the minimal content of the ciliated cells (18%), the maximum frequency of the ciliary beat (FCB) -15 Hz and the lack of directional movement of mucus. Within the 1st month of life, the most intensive growth in the number of the ciliated cells and their morphometric parameters (the height of the cell, the length of the cilia) was marked together with the formation of the pseudostratified structure of the epithelium. By the end of this period morphologically identifiable goblet cells first appeared. The emergence of mucociliary transport (0.03 mm/s) was registered since Day 14 after birth. Tracheal epithelium reached structural and functional maturity by the 3rd month of postnatal development, at this time, the velocity of mucus movement (VMM) increased to 0.1 mm/s, FCB at the age of 6-12 months was equal to 11.3-11.7 Hz. In the aged (24 month old) animals FCB increased to 12.9 Hz, while the morphometric parameters of the ciliated cells, the proportion of goblet cells and VMM reached their maximum values. At the same time, the number of the basal (cambial) cells in the epithelial layer was reduced, while the number of dying cells was increased. The results of the study indicate a close relationship between processes of structural and functional differentiation of the ciliated epithelium of the respiratory tract ensuring the effective operation of mucociliary apparatus throughout the life.
Full Text
About the authors
A. V. Pavlov
Yaroslavl’ State Medical Academy
Email: pavlov@yma.ac.ru
L. I. Yesev
Yaroslavl’ State Medical Academy
References
- Западнюк И. П., Западнюк В. И. и Захария Е. А. Лабораторные животные. Разведение, содержание и использование в эксперименте. Киев, Вища школа, 1974.
- Катинас Г. С. и Быков В. Л. Метод естественной периодизации прoцессов. Арх. анат., 1976, т. 71, вып. 4, с. 98-103.
- Павлов А. В. и Есев Л. И. Методические подходы к комплексному изучению функциональной морфологии эпителиальной выстилки трахеи в эксперименте. Морфология, 2012, т. 142, вып. 6, с. 73-76.
- Шубникова Е. А. Система эпителиальных тканей. В кн.: Руководство по гистологии. 2-е изд. СПб, СпецЛит, 2011, т. 1, с. 124-202.
- Bailey K. L., Bonasera S. J., Wilderdyke M. et al. Aging causes a slowing in ciliary beat frequency, mediated by PKCε. Am. J. Physiol. Lung Cell Mol. Physiol., 2014, v. 306, № 6, p. L584- L589.
- Carson J.L., Collier A.M., Gambling T.M. and Hu S.C. An autoradiographic assessment of epithelial cell proliferation and postnatal maturation of the tracheal epithelium in infant ferrets. Anat. Rec., 1999, v. 256, № 3, p. 242-251.
- Francis R.J., Chatterjee B., Loges N.T. et al. Initiation and maturation of cilia-generated flow in newborn and postnatal mouse airway. Am. J. Physiol. Lung Cell Mol. Physiol., 2009, v. 296, № 6, p. L1067-L1075.
- Ho J.C., Chan K.N., Hu W.H. et al. The effect of aging on nasal mucociliary clearance, beat frequency, and ultrastructure of respiratory cilia. Am. J. Respir. Crit. Care Med., 2001, v. 163, № 4, p. 983-988.
- Joki S. and Saano V. Ciliary beat frequency at six levels of the respiratory tract in cow, dog, guinea-pig, pig, rabbit and rat. Clin. Exp. Pharmacol. Physiol., 1994, v. 21, № 5, p.427-434.
- Joki S. and Saano V. Influence of ageing on ciliary beat frequency and on ciliary response to leukotriene D4 in guinea-pig tracheal epithelium. Clin. Exp. Pharmacol. Physiol., 1997, v. 24, № 2, p. 166-169.
- Kawamata S. and Fujita H. Fine structural aspects of the development and aging of the tracheal epithelium of mice. Arch. Histol. Jpn., 1983, v. 46, № 3, p. 355-372.
- Leigh M.W., Gambling T.M., Carson J.L. et al. Postnatal development of tracheal surface epithelium and submucosal glands in the ferret. Exp. Lung. Res., 1986, v. 10, № 2, p. 153-169.
- Lim M., Elfman F., Dohrman A. et al. Upregulation of the 72-kDa type IV collagenase in epithelial and stromal cells during rat tracheal gland morphogenesis. Dev. Biol., 1995, v. 171, № 2, p. 521-530.
- O’Callaghan C., Smith K., Wilkinson M. et al. Ciliary beat frequency in newborn infants. Arch. Dis. Child., 1991, v. 66, № 4, p. 443-444.
- Smolich J.J., Stratford B.F., Maloney J.E. and Ritchie B.C. Post natal development of the epithelium of larynx and trachea in the rat: scanning electron microscopy. J. Anat., 1977, v. 124, № 3, p. 657-673.
- Voter K.Z., Leigh M.W., Boat T.F. et al. Development of mucociliary transport in the postnatal ferret trachea. J. Appl. Physiol., 1992, v. 73, № 4, p. 1500-1503.