THE CHANGES OF THE INTERRELATIONS OF THE PINEAL GLAND AND THE ORGANS OF THE IMMUNE SYSTEM IN RATS IN RESPONSE TO MELATONIN ADMINISTRATION IN LIGHT REGIME DISTURBANCES



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Abstract

In this work the correlation analysis was applied to detect the integrated response of the pineal gland (PG) and immunocompetent organs of male Wistar rats in response to administration of melatonin (MT) in light regime disturbances. Animals were kept for 14 days under natural or continuous light (CL). Then for 7 days they received the injections of either 0.9% solution of sodium chloride or MT, after which the rats were decapitated and the mass of their body, PG, thymus and spleen was determined. The lymphocyte subpopulations of the thymus and spleen were studied by flow cytometry. The amount of lipofuscin in PG was assessed by the intensity of autofluorescence in organ frozen sections in 560-600 nm wavelength range. It was found that under the influence of MT, the number of intraorgan correlations in the immune system increased, regardless of the light regime. In animals on CL treated with MT, the number of interorgan connections was reduced, while negative correlations appeared between PG lipofuscin content and cellular composition of the spleen. The synchronizing and adaptogenic effects of MT were most pronounced under conditions of CL

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About the authors

G. I. Litvinenko

RAS Siberian Branch Research Institute of Physiology and Fundamental Medicine

Email: litv_g_i@mail.ru
Laboratory of Chronophysiology

O. B. Gritzyk

RAS Siberian Branch Research Institute of Physiology and Fundamental Medicine

Laboratory of Chronophysiology

Ye. V. Mel’nikova

RAS Siberian Branch Research Institute of Physiology and Fundamental Medicine

Laboratory of Chronophysiology

P. A. Avrorov

RAS Siberian Branch Research Institute of Physiology and Fundamental Medicine

Laboratory of Chronophysiology

M. V. Tenditnik

RAS Siberian Branch Research Institute of Physiology and Fundamental Medicine

Laboratory of Chronophysiology

A. V. Shurlygina

RAS Siberian Branch Research Institute of Physiology and Fundamental Medicine

Laboratory of Chronophysiology

A. Alekseevich Trufakin

RAS Siberian Branch Research Institute of Physiology and Fundamental Medicine

Laboratory of Chronophysiology

References

  1. Ветлугина Т. П., Никитина В. Б., Невидимова Т. И. и др. Cистема иммунитета и уровень тревожности при адаптации человека к новым условиям жизнедеятельности // Фундаментальные исследования. 2012. № 9 (часть 1). С. 17-21.
  2. Лебедев К. А., Понякина И. Д. Иммунограмма в клинической практике. М.: Наука, 1990.
  3. Линькова И. С., Полякова В. О., Кветной И. М. Единый механизм ремоделирования межклеточного матрикса в ткани тимуса и эпифиза при старении // Успехи геронтологии. 2011. Т. 24, № 3. С. 420-422.
  4. Литвиненко Г. И., Шурлыгина А. В., Вербицкая Л. В. и др. Суточная динамика клеточного состава тимуса и лимфоузлов мышей в норме, при постоянном освещении и при введении мелатонина // Бюл. экспер. биол. 2005. Т. 140, № 8. С. 181-185.
  5. Литвиненко Г. И., Шурыгина А. В., Грицык О. Б. и др. Взаимоотношения морфофункциональных показателей шишковидной железы и органов иммунной системы у крыс при естественном световом режиме и круглосуточном освещении // Морфология. 2014. Т. 145, вып. 2. С. 26-30.
  6. Ширинский В. С., Непомнящих В. М., Леонова М. И. и др. Иммунофармакодинамика и эффективность гормона эпифиза мелатонина у больных атопическим дерматитом // Росс. иммунол. журн. 2008. Т. 2, № 11. С. 221.
  7. Ahmad R., Haldar C. Photoperiodic regulation of MT1 and MT2 melatonin receptor expression in spleen and thymus of a tropical rodent Funambulus pennanti during reproductively active and inactive phases // Chronobiol. Int. 2010. Vol. 27, № 3. P. 446-462.
  8. Borugian M. J., Gallagher R. P., Friesen M. C. et al. Twenty-fourhour light exposure and melatonin levels among shift workers // J. Occup. Environ. Med. 2005. Vol. 47, № 12. P. 1268-1275.
  9. Doublea K. L., Dedova V. N., Fedorow H. et al. The comparative biology of neuromelanin and lipofuscin in the human brain // Cell. Mol. Life Sci. 2008. Vol. 65. P. 1669-1682.
  10. Esquifino A. I., Pandi-Perumal S. R., Cardinali D. P. Circadian organization of the immune response: A role for melatonin // Clin. Appl. Immunol. Rev. 2004. Vol. 4, № 6. P. 423-433.
  11. Guerrero J. M., Reiter R. J. Melatonin-immune system relationships // Curr. Top. Med. Chem. 2002. № 2. P. 167-179.
  12. Maharaj D. S., Glass B. D., Daya S. Melatonin: new places in therapy // Biosci. Rep. 2007. Vol. 27, № 6. P. 299-320.
  13. Marmorstein A. D., Marmorstein L. Y., Sakaguchi H., Holly-field J. G. Spectral profiling of autofluorescence associated with lipofuscin, bruch’s membrane, and sub-RPE deposits in normal and AMD eyes // Invest. Ophthalmol. Vis. Sci. 2002. Vol. 43, № 7. P. 2435-2441.
  14. Tamura H., Takasaki A., Taketani T. et al. Melatonin and female reproduction // J. Obstet. Gynaecol. Res. 2014. Vol. 40, № 1. P. 1-11.

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