SPREADING OF TISSUE SPHEROIDS FROM PRIMARY HUMAN FIBROBLASTS ON THE SURFACE OF MICROFIBROUS ELECTROSPUN POLYURETHANE MATRIX (A scanning electron microscopic study)



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Abstract

Tissue spheroids biofabricated from primary human fibroblasts using non-adhesive agarose forms, were placed by 3D bioprinter on the surface of microfibrous electrospun matrix. It was demonstrated that tissue spheroids attached to the surface of matrix during several hours and then gradually spread for several days which indicates high level of biocompatibiity of electrospun microfibrous polyurethane matrix. During this activity, human fibroblasts used processes of leading cell borders for initial step of attachment to matrix filaments. Tissue constructions formed during spreading of tissue spheroids on the surface of electrospun microfibrous polyurethane matrix seem to be a perspective technology platform for development of new methods of biofabrication and 3D bioprinting.

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About the authors

Ye. V. Kudan

3D Bioprinting Solutions

Laboratory of Biotechnological Research

F. D. A. S. Pereira

3D Bioprinting Solutions

Laboratory of Biotechnological Research

V. A Parfenov

3D Bioprinting Solutions

Laboratory of Biotechnological Research

V. A Kasyanov

P. Stradina Riga University; Riga Technical University

Email: kasyanov@latnet.lv
Laboratory of Biomechanics

Yu. D. Khesuani

3D Bioprinting Solutions

Laboratory of Biotechnological Research

Ye. A. Bulanova

3D Bioprinting Solutions

Laboratory of Biotechnological Research

A. Aleksandrovich Mironov

3D Bioprinting Solutions

Laboratory of Biotechnological Research

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