Email this article NOVEL CALRETININ-POSITIVE CELLSWITH POLYMORPHOUS SPINES IN MOUSEFOREBRAIN DURING EARLY POSTNATALONTOGENESIS
- Authors: Revishchin AV1, Okhotin VE1, Pavlova GV1, Revishchin AV2, Okhotin VY.2, Pavlova GV2
-
Affiliations:
- RAS Institute of Gene Biology
- Issue: Vol 136, No 6 (2009)
- Pages: 20-27
- Section: Articles
- URL: https://j-morphology.com/1026-3543/article/view/399188
- DOI: https://doi.org/10.17816/morph.399188
- ID: 399188
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Abstract
Using an immunocytochemical method for calretinin (CR)
detection, we have earlier described (Morfologiya, 2009 v. 135, № 3, p. 7-19) the population of previously unknown mono- and bipolar cells with polymorphous spines (PS) covering their cell
bodies and processes, in adult mice forebrain structures adjacent
to anterior horn of lateral ventricle. CR-positive spiny (CR+PS)
cells were negative to GAD67 and were detected in the white
matter and in layers V and VI of frontal area of dorsomedial cortex
close to the cingulum, in in rostro-dorsal part of the caudate
nucleus-putamen complex, anterior olfactory nucleus and in subependymal
layer of the dorso-lateral angle of the lateral ventricle.
In this work, the distribution of these cells in 7-day-old mice was studied. Comparative topographical analysis of definitive and early CR+PS cells demonstrated that in 7-day-old mice CR+PS
cells were absent from the areas of their localization in adult animals
- anterior olfactory nucleus, cortical plate and inner portion
of neostriatum. Meanwhile, some CR+PS-like cells were detected
in 7-day-old mice inside the rostral migratory route, close to neostriatum anterior boundary, along the dorsal border between
neostriatum and corpus callosum, subependymal layer of lateral
wall of the lateral ventricle, and in the cingulum area. These findings
are indicative of the possible postnatal appearance of CR+PS
cells. To test this hypothesis, the experiments were conducted in which bromodeoxyuridine (BrdU) was administered to the mice
on their postnatal days 2-4 with the subsequent study of the brain
sections of these animals sacrificed on their postnatal day 20.
Double immunolabeling of these sections for CR and BrdU has detected the presence of CR+PS cells that contained postnatally
administered BrdU. These results strongly suggest that, at least, some portion of CR+PS cells have their mitosis postnatally. It may be assumed, that CR-PS cells migrate to the sites of their
distribution in adult animals from day 7 till day 20 of postnatal
develpment.
detection, we have earlier described (Morfologiya, 2009 v. 135, № 3, p. 7-19) the population of previously unknown mono- and bipolar cells with polymorphous spines (PS) covering their cell
bodies and processes, in adult mice forebrain structures adjacent
to anterior horn of lateral ventricle. CR-positive spiny (CR+PS)
cells were negative to GAD67 and were detected in the white
matter and in layers V and VI of frontal area of dorsomedial cortex
close to the cingulum, in in rostro-dorsal part of the caudate
nucleus-putamen complex, anterior olfactory nucleus and in subependymal
layer of the dorso-lateral angle of the lateral ventricle.
In this work, the distribution of these cells in 7-day-old mice was studied. Comparative topographical analysis of definitive and early CR+PS cells demonstrated that in 7-day-old mice CR+PS
cells were absent from the areas of their localization in adult animals
- anterior olfactory nucleus, cortical plate and inner portion
of neostriatum. Meanwhile, some CR+PS-like cells were detected
in 7-day-old mice inside the rostral migratory route, close to neostriatum anterior boundary, along the dorsal border between
neostriatum and corpus callosum, subependymal layer of lateral
wall of the lateral ventricle, and in the cingulum area. These findings
are indicative of the possible postnatal appearance of CR+PS
cells. To test this hypothesis, the experiments were conducted in which bromodeoxyuridine (BrdU) was administered to the mice
on their postnatal days 2-4 with the subsequent study of the brain
sections of these animals sacrificed on their postnatal day 20.
Double immunolabeling of these sections for CR and BrdU has detected the presence of CR+PS cells that contained postnatally
administered BrdU. These results strongly suggest that, at least, some portion of CR+PS cells have their mitosis postnatally. It may be assumed, that CR-PS cells migrate to the sites of their
distribution in adult animals from day 7 till day 20 of postnatal
develpment.
About the authors
A V Revishchin
Email: ve_okhotin@mail.ru
V E Okhotin
Email: ve_okhotin@mail.ru
G V Pavlova
Email: ve_okhotin@mail.ru
A V Revishchin
RAS Institute of Gene BiologyRAS Institute of Gene Biology
V Ye Okhotin
RAS Institute of Gene BiologyRAS Institute of Gene Biology
G V Pavlova
RAS Institute of Gene BiologyRAS Institute of Gene Biology
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