Email this article REACTIVITY MECHANISMS OF AIRWAYSMOOTH MUSCLE TISSUE IN EXPERIMENTALBRONCHIAL SPASM
- Authors: Zashikhin AL1, Agafonov Y.V1, Barmina AO1, Zashikhin AL2, Agafonov Y.V2, Barmina AO2
-
Affiliations:
- NorthernState Medical University
- Issue: Vol 136, No 6 (2009)
- Pages: 69-74
- Section: Articles
- URL: https://j-morphology.com/1026-3543/article/view/399218
- DOI: https://doi.org/10.17816/morph.399218
- ID: 399218
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Abstract
This investigation was aimed at the complex evaluation of the reactivity mechanisms of bronchial smooth muscle tissue (SMT)
in experimental bronchial spasm. Morphometric, cytospectrophotometric
and electron microscopical analysis demonstrated
the presence of three types of smooth muscle cells (SMC) within
the bronchial SMT (small, medium, large), that differed in their
linear and metabolic parameters. The findings of this study indicate
that under the conditions of experimental bronchial spasm
development, the ratios of SMC in bronchial SMT are changed
with the increase in proportion of small SMC and the elimination
of large SMC. In the dynamics of experimental bronchial spasm
development, the activation of cytoplasmic synthesis as well as of DNA synthesis was detected mainly in group of small SMC.
The reactive-dystrophic changes were marked at the subcellular
level, that were most often identified in large SMC resulting in their elimination from population in the dynamics of an experiment.
The data obtained suggest that one of the important mechanisms
of airway SMT adaptation to the bronchial spasm development
is a dynamic reorganization of SMC population.
in experimental bronchial spasm. Morphometric, cytospectrophotometric
and electron microscopical analysis demonstrated
the presence of three types of smooth muscle cells (SMC) within
the bronchial SMT (small, medium, large), that differed in their
linear and metabolic parameters. The findings of this study indicate
that under the conditions of experimental bronchial spasm
development, the ratios of SMC in bronchial SMT are changed
with the increase in proportion of small SMC and the elimination
of large SMC. In the dynamics of experimental bronchial spasm
development, the activation of cytoplasmic synthesis as well as of DNA synthesis was detected mainly in group of small SMC.
The reactive-dystrophic changes were marked at the subcellular
level, that were most often identified in large SMC resulting in their elimination from population in the dynamics of an experiment.
The data obtained suggest that one of the important mechanisms
of airway SMT adaptation to the bronchial spasm development
is a dynamic reorganization of SMC population.
Keywords
About the authors
A L Zashikhin
Email: zashihin2@atknet.ru
Yu V Agafonov
A O Barmina
Email: barminastya@yandex.ru
A L Zashikhin
NorthernState Medical UniversityNorthernState Medical University
Yu V Agafonov
NorthernState Medical UniversityNorthernState Medical University
A O Barmina
NorthernState Medical UniversityNorthernState Medical University
References
- Агроскин Л.С. и Папаян Г.В. Цитофотометрия. Л., Наука, 1977.
- Брумберг В.А. и Певзнер Л.З. Использование амидочерного в цитофотометрическом исследовании клеточных протеинов. Цитология, 1972, т. 14, № 5, с. 674-676.
- Зашихин А.Л., Агафонов Ю.В. и Лисишников Л.В. Патент РФ на изобретение № 2104524 от 23.05.1994 г. «Способ получения препаратов изолированных клеток».
- Зашихин А.Л. и Селин Я. Висцеральная гладкая мышечная ткань. Архангельск, Умеа, изд. СГМУ, 2001.
- Пирс Э. Гистохимия теоретическая и прикладная. М., Издво иностр. лит-ры, 1962.
- Хесин Я.Е. Размеры ядер и функциональное состояние клеток. М., Медицина, 1967.
- Barnes P.J. and Cosio M.G. Cells and mediators of chronic obstructive pulmonary disease. Eur. Respir. Monograph, 2006, v. 38, p. 130-158.
- Doherty T. and Broid D. Cytokines and growth factors in airway remodeling in asthma. Curr. Opin. Immunol., 2007, v. 19, № 6, p. 676-680.
- Gabella G. Development of visceral smooth muscle. Results Probl. Cell Differ., 2002, v. 38, p. 1-37.
- Giuriato L.E., Chiavegato A. and Pauletto P. Correlation between the presence of an immature smooth muscle cell population in tunica media and the development of atherosclerotic lesion. A study on different-sized rabbit arteries from cholesterol-fed and Watanabe heritable hyperlipemic rabbits. Atherosclerosis, 1995, v. 116, № 1, p. 77-92.
- Groves G., Wang Z. and Newman W.H. Two distinct phenotypes of rat vascular smooth muscle cells:growth rate and production of tumor necrosis factor-alpha. Am. Surg., 2005, v. 71, № 7, p. 546-550.
- Halayko A.J. and Amrani Y. Mechanisms of inflammation-mediated airway smooth muscle plasticity and airways remodelling in asthma. Respir. Physiol. Neurobiol., 2003, v. 137, p. 209-222.
- Halayko A.J. and Solway J. Molecular mechanisms of phenotypic plasticity in smooth muscle cells. J. Appl. Physiol., 2001, v. 90, p. 358-368.
- Halayko A.J., Stelmack G.L. and Yamasaki A. Distribution of phenotypically disparate myocytes subpopulations in airway smooth muscle. Can. J. Physiol. Pharmacol., 2005, v. 83, p. 104-116.
- Neylon C.B., Avdonin P.V. and Dilley R.J. Different electrical responses to vasoactive agonists in morphologically distinct smooth muscle cell types. Cir. Res., 1994, v. 75, № 4, p. 733-741.
- Reynolds E.S. The use of lead citrate at high pH as an electronopaque stain in electron microscopy. J. Cell. Biol., 1963, v. 17, p. 208-212.
- Woodruff P.G., Dolganov G.M., Ferrando R.E. et al. Hyperplasia of smooth muscle in mild to moderate asthma without changes in cell size or gene expression. Am. J. Respir. Crit. Care. Med., 2004, v. 169, p. 1001-1006.
- Zashikhin A.L. and Agafonov J.V. Allergen induced airway hyperresponsiveness in guinea pigs: the characteristic feature of smooth muscle population. Allergy, 1996, v. 51, № 32, p. 194.
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СМИ зарегистрировано Федеральной службой по надзору в сфере связи, информационных технологий и массовых коммуникаций (Роскомнадзор).