REACTIVE CHANGES OF SMOOTH MUSCLE TISSUE OF RAT SMALL INTESTINE DURING EXPERIMENTAL INTESTINAL OBSTRUCTION



Cite item

Full Text

Open Access Open Access
Restricted Access Access granted
Restricted Access Subscription or Fee Access

Abstract

Using light, electron microscopy and immunohistochemical methods, the reactive transformation of smooth muscle tissue (SMT) was studied in the intestinal wall during the development of acute partial high intestinal obstruction. The material of small intestine was taken from 10 male rats in both the zone of ligature application, and proximal and distal zones, 3 cm distant from the ligation zone. The results of the study demonstrate that in partial intestinal obstruction, the nature of structural and functional SMT transformation was variable depending upon differences in functional and destructive loads. During these changes, the remodeling of smooth myocyte population was shown to be one of the mechanisms of SMT adaptation to the changing conditions of functioning. Immunohistochemical analysis found no changes in the pattern of expression of marker and phenotypic proteins in the intestinal zones studied during the dynamics of an experiment.

References

  1. Брумберг В.А. и Певзнер Л.З. Использование амидочерного в цитофотометрическом исследовании клеточных протеинов. Цитология, 1972, т. 14, № 5, с. 674-676.
  2. Гладкий А.П. и Баженов Д.В. Реактивность гладкой мышечной ткани толстой кишки в условиях экспериментальной непроходимости. Арх. анат., 1976, т. 71, вып. 7, с. 47-51.
  3. Зашихин А.Л. и Агафонов Ю.В. Структура популяции гладких миоцитов (аспекты внутриорганной организации гладкой мышечной ткани). Морфология, 1997, т. 112, вып. 4, с. 61-67.
  4. Зашихин А.Л., Агафонов Ю.В. и Лисишников Л.В. Патент РФ на изобретение № 2104524 от 23.05.1994 г. «Способ получения препаратов изолированных клеток».
  5. Кауфман О.Я. Гладкая мышечная ткань. В кн.: Структурные основы адаптации и компенсации нарушений функций. М., Медицина, 1987, с. 131-153.
  6. Пирс Э. Гистохимия теоретическая и прикладная. М., Изд-во иностр. лит-ры, 1962.
  7. Хесин Я.Е. Размеры ядер и функциональное состояние клеток. М., Медицина, 1967.
  8. Bertoni S., Gabella G., Ballabeni V. et al. Plasticity of rat small intestine after removal of a chronic mechanical obstruction. Neurogastroenterol. Motil., 2006, v. 18, p. 862-872.
  9. Cogliandro R.F., De Giorgio R., Barbara G. et al. Chronic intestinal pseudo-obstruction. Best Pract. Res. Clin. Gastroenterol., 2007, v. 21, № 4, p. 657-669.
  10. Dite P., Lata J. and Novotny I. Intestinal obstruction and perforation - the role of the gastroenterologist. Dig. Dis., 2003, v. 21, № 1, p. 63-67.
  11. Gabella G. Development of visceral smooth muscle. Results Probl. Cell Differ., 2002, v. 38, p. 1-37.
  12. Guarino M.P., Carotti S., Cogliandro R. et al. Impaired contractility of colonic muscle cells in a patient with chronic intestinal pseudo-obstruction. Dig. Liver Dis., 2007, v. 10, p. 225 229.
  13. Huizinga J.D. Neural injury, repair and adaptation in the GI tract. IV Pathophysiology of GI motility related to interstitial cells of Cajal. Am. J. Physiol., 1998, v. 275 (3 Pt 1), p. 381-396.
  14. Paulin D. and Li Z. Desmin: a major intermediate filament protein essential for the structural integrity and function of muscle. Exp. Cell Res., 2004, v. 301, p. 1-7.
  15. Stanghellini V., Cogliandro R.F., de Giorgio R. et al. Chronic intestinal pseudo-obstruction: manifestations, natural history and management. Neurogastroenterol. Motil., 2007, v. 19, № 6, p. 440-452.
  16. Reynolds E.S. The use of lead citrate at high pH as an electronopaque stain in electron microscopy. J. Cell Biol., 1963, v. 17, p. 208-212.
  17. Won K-J., Suzuki T., Hori M. et al. Motility disorder in experimentally obstructed intestine: relationship between muscularis inflammation and disruption of the ICC network. Neurogastroenterol. Motil., 2006, v. 18, p. 53-61.

Supplementary files

Supplementary Files
Action
1. JATS XML
Download

Copyright (c) 2010 Eco-Vector


СМИ зарегистрировано Федеральной службой по надзору в сфере связи, информационных технологий и массовых коммуникаций (Роскомнадзор).
Регистрационный номер и дата принятия решения о регистрации СМИ: № 0110212 от 08.02.1993.

This website uses cookies

You consent to our cookies if you continue to use our website.

About Cookies