MORPHOLOGICAL PARAMETERS OF LIVER IN RATS DURING THE CHANGES OF NITRIC OXIDE CONTENT AND DEAFFERENTATION WITH CAPSAICIN



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Abstract

The aim of this investigation was to study the morphological changes of rat liver during the blockade of nitric oxide (NO) synthesis and its increased content in the intact rats and after the damage of capsaicin-sensitive nerves. It was shown that the damage of capsaicin-sensitive nerves resulted in dystrophic and necrotic changes in the liver, associated with the inflammatory reaction. These changes were enhanced by the blockade of NO-synthase. NO deficiency, caused by NO-synthase blockade, lead to disturbances of vascular bed control, resulting in ischemia and hypoxia, and, as a consequence, in dystrophic and necrotic changes in hepatocytes. The data obtained indicate that in the development of dystrophic changes after the modification of the function of capsaicin-sensitive nerves, along with the decrease in the sensory neuropeptide content, the significant role is played by NO-dependent machanisms. Administration of NO precursor, L-arginine, after deafferentation with capsaicin, inhibited the inflammatory reactions in the liver.

References

  1. Автандилов Г.Г. Медицинская морфометрия. М., Медицина, 1990.
  2. Chu C.J., Chang C.C. and Wang T.F. Detrimental effects of nitric oxide ingibition on hepatic encephalopathy in rats with thioacetamide-induced fulminant hepatic failure: role of nitric oxide synthase isoforms. J. Gastroenterol. Hepatol., 2006, v. 21, № 7, p. 1194-1199.
  3. Curran J.N., Winter D.C. and Bouchier-Hayes D. Biological fate and clinical implications of arginine metabolism in tissue healing. Wound Repair Regen., 2006, v. 14, № 4, p. 376-386.
  4. Filep J.G., Foldes-Filep E., Rousseau A. et al. Vascular responses to endothelin-1 following inhibition of nitric oxide synthesis in the conscious rat. Br. J. Pharmacol., 1993, v. 110, № 3, p. 1213-1221.
  5. Gabriel A., Porrino M.L., Stephenson L.L. and Zamboni W.A. Effect of L-arginine on leukocyte adhesion in ischemia-reperfusion injury. Plast. Reconstr. Surg., 2004, v. 113, № 6, p. 1698- 1702.
  6. Holzer P., Wachter C., Heinemann A. et al. Sensory nerves, nitric oxide and NANC vasodilatation. Arch. Int. Pharmacodyn Ther., 1995, v. 329, № 1, p. 67-69.
  7. Loehe F., Bruns C.J., Nitsch S.M. et al. The role of L-arginine following trauma and blood loss. Curr. Opin. Clin. Nutr. Metab. Care., 2007, v. 10, № 1, p. 80-87.
  8. Merhi M., Dusting G.J. and Khalil Z. CGRP and nitric oxide of neuronal origin and their neurogenic vasodilatation in rat skin microvasculature. Br. J. Pharmacol., 1998, v. 123, p. 863-868.
  9. Ou J., Carlos T.M., Watkins S.C. et al. Differential effects of nonselective nitric oxide synthase (NOS) and selective inducible NOS inhibition on hepatic necrosis, apoptosis, ICAM-1 expression, and neutrophil accumulation during endotoxemia. Nitric. Oxide, 1997, v. 5, № 1, p. 404-416.
  10. Pacher P., Beckman J.S. and Liaudet L. Nitric oxide and peroxynitrite in health and disease. Phisiol. Rev., 2007, v. 87, № 1, p. 315-424.
  11. Peitan L., Yin K., Nagele R. et al. Inhibition of nitric oxide synthase attenuates peroxynitrite generation, but augment neutrophil accumulation in hepatic ischemia-reperfusion in rats. Pharmacology, 1998, v. 284, № 3, p. 1139-1146.
  12. Szolcsamyi J. Forty years in capsaicin research for sensory pharmacology and physiology. Neuropeptides, 2004, v. 38, № 6, p. 377-384.
  13. Thippeswamy T. and Morris R. The role of nitric oxide in dorsal root ganglion neurons. Ann. N.Y. Acad. Sci., 2002, v. 962, p. 103-110.
  14. Zheng Z., Shimamura K., Anthony T.L. et al. Nitric oxide is a sensory nerve neurotransmitter in the mesenteric artery of guinea pig. J. Auton. Nerv. Syst., 1997, v. 67, № 3, p. 137-144.

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