CHANGES OF THE TISSUES AND OF REGIONAL LYMPH NODES IN RATS WITH CHRONIC INFLAMMATORY PROCESS TREATED WITH INTERLEUKIN-2



Cite item

Full Text

Open Access Open Access
Restricted Access Access granted
Restricted Access Subscription or Fee Access

Abstract

The structure of Wistar rat subcutaneous adipose tissue and axillary (regional) lymph nodes (LN) was studied by the methods of light microscopy after the subcutaneous implantation of the fragments of silicone mammary implant in the area of right scapula in animals treated with interleukin-2 (IL-2). Subcutaneous implantation of sterile silicone plates to rats was found to induce chronic granulomatous inflammatory process with the formation of connective tissue capsule. Implants placement to rats treated with IL-2 resulted in the formation of a thicker capsule with the signs of sclerosis, fibrosis and hyialinosis; all the capsular structures were heavily infiltrated with macrophages, which contained phagocytozed silicone fragments. After the introduction of silicone implants, the volume of the capsule and connective tissue in the regional LN progressively increased, while after IL-2 treatment the intensity of sclerotic processes was significantly decreased. The relative area of lymphoid nodules without the germinal centers in LN sections was increased 1 month following the induction of chronic inflammatory process in rats treated with IL-2, but by 6 months the proportion of nodules both with or without germinal centers become less, as compared to the initial data.

References

  1. Добрякова О.Б. и Ковынцев Н.Н. Аугментационная маммопластика силиконовыми эндопротезами. М., МОК ЦЕНТР, 2000.
  2. Златник Е.Ю. и Голотина Л.Ю. Применение ронколейкина для ЛАК-терапии рака яичника. Вопр. онкол., 2005, т. 51, № 6, с. 680-684.
  3. Майбородин И.В., Гаврилова В.В., Колмакова И.А. и др. Возрастные и половые особенности тканей десны в норме и при деструктивном хроническом периодонтите. Стоматология, 2009, т. 88, № 2, с. 29-33.
  4. Майбородин И.В., Ковынцев Н.Н. и Добрякова О.Б. Нарушения микроциркуляции как причина капсулярной контрактуры после увеличивающей маммопластики. Хирургия, 2007, № 3, с. 49-53.
  5. Accurso A., Rocco N., Feleppa C. et al. Spread of silicone to axillary lymph nodes after high cohesive gel silicone implant rupture. Plast. Reconstr. Surg., 2008, v. 122, № 6, p. 221e-222e.
  6. Dillman R.O. Lymphocyte therapy of renal cell carcinoma. Expert. Rev. Anticancer Ther., 2005, v. 5, № 6, p. 1041-1051.
  7. Ersek R.A. and Beisang A.A. 3rd. Bioplastique: a new textured copolymer microparticle promises permanence in soft-tissue augmentation. Plast. Reconstr. Surg., 1991, v. 87, № 4, p. 693- 702.
  8. Gayou R. and Rudolph R. Capsular contraction around silicone mammary prostheses. Ann. Plast. Sur., 1979, v. 2, № 1, p. 62-71.
  9. Hancock W.W., Kobzik L., Colby A.J. et al. Detection of lymphokines and lymphokine receptors in pulmonary sarcoidosis. Immunohistologic evidence that inflammatory macrophages express IL-2 receptors. Am. J. Pathol., 1986, v. 123, № 1, p. 1-8.
  10. Khan U.D. Left unilateral breast autoinflation and intraprosthetic collection of sterile pus: an unusual operative finding of silicone gel bleed with silicone lymphadenitis. Aesthetic Plast. Surg., 2008, v. 32, № 4, p. 684-687.
  11. Kjoller K., Holmich L.R., Jacobsen P.H. et al. Capsular contracture after cosmetic breast implant surgery in Denmark. Ann. Plast. Surg., 2001, v. 47, № 4, p. 359-366.
  12. Laitung J.K., McClure J. and Shuttleworth C.A. The fibrous capsules around static and dynamic implants: their biochemical, histological, and ultrastructural characteristics. Ann. Plast. Surg., 1987, v. 19, № 3, p. 208-216.
  13. Malek T.R., Yu A., Zhu L., Matsutani T. et al. IL-2 family of cytokines in T regulatory cell development and homeostasis. J. Clin. Immunol., 2008, v. 28, № 6, p. 635-639.
  14. Marguti I., Yamamoto G.L., Costa da T.B. et al. Expansion of CD4+ CD25+ Foxp3+ T cells by bone marrow-derived dendritic cells. Immunology, 2009, v. 127, № 1, p. 50-61.
  15. Muramatsu Y., Sugino K., Kikuchi N. et al. Human adjuvant disease which developed after silicone augmentation mammoplasty. Nihon Kokyuki Gakkai Zasshi, 2009, v. 47, № 3, p. 237-241.
  16. Pierce V.E. Jr., Pantazis C.G. and Ades E.W. Examination of differentiation, proliferation and distribution of lymphoid cells in mice by immunohistochemical analysis post in vivo administration of recombinant interleukin-II. J. Clin. Lab. Immunol., 1988, v. 25, № 1, p. 47-52.
  17. Piguet P.F., Grau G., Irle C. and Vassalli P. Administration of recombinant interleukin 2 to mice enhances production of hemopoietic and natural killer cells. Eur. J. Immunol., 1986, v. 16, № 10, p. 1257-1261.
  18. Poudrier J. and Owens T. Th1 and Th2 help for B cells: differential capacity for induction of autonomous responsiveness to IL-2. Int. Immunol., 1995, v. 7, № 6, p. 1021-1027.
  19. Spieker-Polet H., Yam P.C., Arbieva Z. et al. In vitro induction of the expression of multiple IgA isotype genes in rabbit B cells by TGF-beta and IL-2. J. Immunol., 1999, v. 162, № 9, p. 5380-5388.
  20. Wang Z.H., Zhu J.Y., Li T. and Leng X.S. Proliferation of CD4+ CD25+ regulatory T cells of rat by different cytokines in vitro. Zhonghua Yi Xue Za Zhi, 2008, v. 88, № 12, p. 844-847.

Supplementary files

Supplementary Files
Action
1. JATS XML
Download

Copyright (c) 2011 Eco-Vector


СМИ зарегистрировано Федеральной службой по надзору в сфере связи, информационных технологий и массовых коммуникаций (Роскомнадзор).
Регистрационный номер и дата принятия решения о регистрации СМИ: № 0110212 от 08.02.1993.

This website uses cookies

You consent to our cookies if you continue to use our website.

About Cookies