REPARATIVE OSTEOGENESIS AFTER TRANSPLANTATION OF BONE MARROW MULTIPOTENT STROMAL CELLS CULTIVATED AT VARIOUS OXYGEN CONCENTRATIONS



Cite item

Full Text

Open Access Open Access
Restricted Access Access granted
Restricted Access Subscription or Fee Access

Abstract

Comparative histological and morphometric methods were used to study the bone callus (BC) in rats 14 and 30 days after the experimental fibula fracture. Animals were infused with cell preparations of multipotent bone marrow stromal cells - BMSC (also known as multipotent mesenchymal stromal cells) in the site of injury immediately after the fracture. BMSC were cultivated in vitro under normoxic (20% O2) and hypoxic (5% O2) conditions. 14 days after the fracture, in rats that received no BMSC (control group) and in animals injected with BMSC, newly formed BC contained fibrous tissue, cartilage and reticulofibrous bone tissue (RFBT). The portion of BC, occupied by RFBT was significantly greater in rats that received BMSC grown at 5%O2, than in the other experimental groups. Thickening index of BC at day 14 was 1.3 and 1.4 times higher in animals treated with BMSC grown at 5% and 20%O2 (p<0.05) than in rats that received no BMSC. At day 30, BC was histologically more mature in rats that received BMSC infusion than in the control group, while the restoration of the initial bone thickness was also more effective in these animals. Thus, the results of this study demonstrated that the infusion of allogeneic BMSC, expanded in vitro at different oxygen concentrations, into the site of fracture improved osteocartilaginous fragment and BC formation and bone size restoration in rats with fibula fracture.

References

  1. Буравкова Л.Б. и Анохина Е.Б. Влияние гипоксии на стромальные клетки-предшественники из костного мозга крыс на ранних этапах культивирования. Бюл. экспер. биол., 2007, т. 143, № 4, с. 386-389.
  2. Буравкова Л.Б., Капланский А.С., Андреева Е.Р. и др. Особенности формирования костной мозоли у крыс после введения в область перелома мультипотентных мезенхимальных стромальных клеток, культивируемых при различном содержании кислорода. Клет. трансп. ткан. инж., 2009, т. 4, № 3, с. 52-57.
  3. Деев Р.В., Цупкина Н.В., Николаенко Н.С. и др. Результаты трансплантации культуры аутогенных стромальных клеток костного мозга в область краевого дефекта длинных трубчатых костей. Травматол. ортопед. России, 2007, т. 2, № 44, с. 57-63.
  4. Дурнова Г.И., Логинов В.И. и Капланский А.С. Заживление перелома малоберцовой кости у крыс при длительном вывешивании. Авиакосм. эколог. мед., 2002, т. 36, № 3, с. 52-55.
  5. Кругляков П.В., Соколова И.Б., Зинькова Н.Н. и др. Влияние сингенных мезенхимных стволовых клеток на восстановление костной ткани у крыс при имплантации деминерализованного костного матрикса. Цитология, 2005, т. 57, № 6, с. 466-477.
  6. Фатхутдинов Т.Х., Гольдштейн Д.В., Пулин А.А. и др. Особенности репаративного остеогенеза при трансплантации мезенхимальных стволовых клеток. Бюл. экспер. биол., 2005, т. 140, № 7, с. 109-113.
  7. Caplan A.I. Adult mesenchymal stem cells for tissue engineering versus regenerative medicine. J. Cell. Physiol., 2007, v. 213, р. 341-347.
  8. Hu X., Yu S.P., Fraser J.L. et al. Transplantation of hypoxiapreconditioned mesenchymal stem cells improves infarcted heart function via enhanced survival of implanted cells and angiogenesis. J. Thorac. Cardiovasc. Surg., 2008, v. 135, № 4, р. 799-808.
  9. Im G.Il., Kim D.Y., Shin J.H. et al. Repair of cartilage defect in the rabbit with cultured mesenchymal stem cells from bone marrow. J. Bone Joint Surg. Br., 2001, v. 83-B, р. 289-294.
  10. Lennon D.P., Edmison J.M. and Caplan A.I. Cultivation of rat marrow-derived mesenchymal stem cells in reduced oxygen tension: effects on in vitro and in vivo osteochondrogenesis. J. Cell Physiol., 2001, v. 187, № 3, р. 345-355.
  11. Malladi P., Xu Y., Chiou M. et al. Effect of reduced oxygen tension on chondrogenesis and osteogenesis in adipose-derived mesenchymal cells. Am. J. Physiol. Cell Physiol., 2006, v. 290, № 4, р. 1139-1146.
  12. Psaltis P.J., Zannettino A.C.W., Worthley S.G. and Grothos S. Concise review: mesenchymal stromal cells: potential for cardiovascular repair. Stem Cells, 2008, v. 26, р. 2201-2210.
  13. Tsuchida H., Hashimoto J., Crawford E. et al. Engineered allogeneic mesenchymal stem cells repair femoral segmental defect in rats. J. Orthop. Res., 2003, v. 2, № 1, р. 44-53.
  14. Wakitani S., Imoto R., Yamamoto T. et al. Human autologous culture expanded bone marrow meenchchimal cell transplantation for repair of cartilage defects in osteoarthritic knees. Osteoarthritis Cartilage, 2002, v. 10, № 3, р. 199-206.

Supplementary files

Supplementary Files
Action
1. JATS XML
Download

Copyright (c) 2011 Eco-Vector


СМИ зарегистрировано Федеральной службой по надзору в сфере связи, информационных технологий и массовых коммуникаций (Роскомнадзор).
Регистрационный номер и дата принятия решения о регистрации СМИ: № 0110212 от 08.02.1993.

This website uses cookies

You consent to our cookies if you continue to use our website.

About Cookies