PROBABLE SOURCES OF ENDOTHELIAL CELLS CIRCULATING IN BLOOD



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Abstract

The aim of study was to determine the probable sources of circulating endothelial cells (CECs) and establish the role of both VE-cadherin and vascular endothelial growth factor (VEGF) in molecular mechanism of endothelium desquamation using the hemorrhagic fever with renal syndrome as the model of endothelium injury. Autopsy pieces of kidney, liver, lung, brain, stomach and myocardium taken from 10 patients were studied histologically. Quantitative measurement of CECs was performed by the method developed by J. Hladovec (1978). Blood levels of VE-cadherin and VEGF were determined by ELISA method. Predominant sources CECs were found to be macro- and microvessels of kidney, liver, lung, brain, gastric mucous membrane, myocardium and, possibly - brain vessels. One of the molecular mechanism of endothelium desquamation is VEGF-stimulated internalization of VE-cadherin.

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