HETEROTOPIC TRANSPLANTATION OF NON-IMMUNOGENIC TRACHEA POPULATED WITH RECIPIENT BONE MARROW STROMAL CELLS



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Abstract

Morphological changes in decellularized allogenic trachea populated with recipient bone marrow stromal (mesenchymal) stem cells and transplanted heterotopically, were examined in 30 C57Bl/6 and Balb/c mice of 22-25 g body mass. The research results have shown the insufficient efficacy of a transplant preparation mode by freezing and thawing method as in this case inflammatory reaction developed in the transplant area and its rejection took place. It was established that the mode of obtaining decellularized tracheal transplant by means of sodium perchlorate (NaClO4) treatment, proposed by the authors, unlike a freezing-thawing mode, allowed to efficiently remove immunocompetent cells that expressed MHC I and II markers. NaClO4 effect did not result in either chondrocyte damage or significant disturbance of tracheal cartilaginous and connective tissue structure in heterotopic transplants. Since transplant population with bone marrow stromal stem cells promoted connective tissue restoration, reduced the formation of granulations in anastomosis area and favored faster transplant epithelization, most promising method of trachea preparation for transplantation apparently seems to be the combination of immune cell removal from this organ by NaClO4 treatment with subsequent bone marrow stromal stem cell population of transplant obtained.

References

  1. Campioni D., Lanza F., Dominici M. et al. Functional and immunophenotypic characteristics of isolated CD105(+) and fibroblast(+) stromal cells from AML: implications for their plasticity along endothelial lineage. Cytotherapy, 2000, v. 5, № 1, p. 566-579.
  2. Hele D.J., Yacoub M.H. and Belvisi M.G. The heterotopic tracheal allograft as an animal model of obliterative bronchiolitis. Respir Res., 2001, v. 2, № 3, p. 169-183.
  3. Hosenpud G.D. The bronchial epithelium: a potential allogenic target for chronic rejection after lung transplantation. J. Heart Lung Transplant., 1996, v 15, № 7, p. 665-674.
  4. Lu K.C., Jaramillo A., Mendeloff E.N. et al. Concomitant allorecognition of mismatched donor HLA class I- and II-derived peptides in pediatric lung transplant recipients with bronchiolitis obliterans syndrome. J Heart Lung Transplant., 2003, v. 22, № 1, p. 35-43.
  5. Martinod E., Seguin A., Radu D. et al. Advances in tracheal surgery: are we close to finding the ideal tracheal substitute? Rev. Mal. Respir., 2010, v. 27, № 6, p. 554-564
  6. Neuringer I.P., Mannon R.B., Coffman T.M. et al. Immune cells in a mouse airway model of obliterative bronchiolitis. Am. J. Respir. Cell Mol. Biol., 1998, v. 19, № 3, p. 379-386.
  7. Nusair S., Or R., Junadi S. et al. Simultaneous donor marrow cell transplantation with reduced intensity conditioning prevents tracheal obliteration in a bronchiolitis obliterans murine model. Chest, 2005, v. 128, № 6, p. 4024-4029.
  8. Ross D.J., Lewis M.I., Kramer M. et al. The impact of cytolytic therapy on bronchiolitis obliterans syndrome. J. Heart Lung Transplant., 1998, v. 17, № 9, p. 869-875.
  9. Sykes M. Immune evasion by chimeric trachea. N. Engl. J. Med., 2010, v. 362, № 2, p. 172-174.
  10. Taylor D.O., Edwards L.B., Mohacsi P.J. et al. The Registry of the International Society for Heart and Lung Transplantation: twentieth official adult lung and heart-lung transplant report. J. Heart Lung Transplant., 2003, № 6, p. 625-639.
  11. Tullius S.G. and Tilney N.L. Both alloantigen-dependent and independent factors influence chronic allograft rejection. Transplantation, 1995, v. 59, № 3, p. 313-318.

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