SYNAPTOGENESIS IN THE DORSAL RAPHE NUCLEUS OF RAT MEDULLA OBLONGATA IN SEROTONIN DEFICIENCY
- Authors: Khozhai L.I.1, Otellin V.A.1
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Affiliations:
- RAS I. P. Pavlov Institute of Physiology
- Issue: Vol 142, No 6 (2012)
- Pages: 020-024
- Section: Articles
- URL: https://j-morphology.com/1026-3543/article/view/399614
- DOI: https://doi.org/10.17816/morph.399614
- ID: 399614
Cite item
Abstract
The purpose of the present study was to examine the synaptogenesis in the dorsal raphe nucleus (DRN) in Wistar rats (n=8–10 for each time point) in the end of prenatal (days 19 and 20) and early postnatal (days 5 and 20) periods, and to determine the role of serotonin in the formation of synaptic contacts during this period of development. It is shown that at prenatal day19 neuropil started to develop in DRN -d, DRN -v and DRN –l in control (intact) animals, while the synaptic contacts appeared on cell processes. At prenatal day20 synaptic contacts were detected for the first time on neuronal cell body surface. The density of synaptophysin-positive granules on both the cell processes in the neuropil, and neuronal cell bodies was sharply and considerably increased by postnatal day 5. Subsequently, until postnatal day 20, the augmentation of their density was insignificant. Serotonin deficiency in a second half of prenatal development, induced by a single injection of parachlorophenylalanine to female rats at gestational day 16 resulted in a significant delay of synaptogenesis in DRN of their offspring in both prenatal and early postnatal periods. With the increase of postnatal age the density synaptic contacts was unequally augmented in various parts of DRN: in DNR-d it approached the control level, while in DRN-v and DRN-l it remained significantly reduced. The results received suggest serotonin participation in synaptogenesis in DRN.
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About the authors
L. I. Khozhai
RAS I. P. Pavlov Institute of Physiology
Email: astarta0505@mail.ru
V. A. Otellin
RAS I. P. Pavlov Institute of Physiology
References
- Отеллин В. А., Хожай Л. И. и Ордян Н. Э. Пренатальное стрессирование и развивающийся мозг. Адаптивные механизмы, непосредственные и отсроченные эффекты. СПб., Десятка, 2007.
- Попова Н. К., Никулина Э. М., Арав В. Н. и Кудрявцева Н. Н. О роли серотонина в одном из видов агрессивного поведения. Физиол. журн. им. И. М. Сеченова, 1975, т. 61, № 2, с. 183–186.
- Семченко В. В., Боголепов Н. Н. и Степанов С. С. Синаптоархитектоника коры большого мозга. Омск, Омич, 1995.
- Хожай Л. И. Формирование астроглии в неокортексе мыши после пренатального временного блокирования синтеза серотонина. Морфология, 2005, т. 127, вып. 1, с. 17–21.
- Хожай Л. И. и Отеллин В. А. Формирование неокортекса у мышей, развивающихся в условиях пренатального дефицита серотонина. Морфология, 2005, т. 127, вып. 3, с. 17–21.
- Хожай Л. И., Отеллин В. А. и Неокесарийский А. А. Постнатальные изменения морфологии нейральных и глиальных клеток в неокортексе мышей, развивавшихся на фоне дефицита серотонина. Журн. эволюц. биохим., 2007, т. 43, № 3, с. 293–298.
- Хожай Л. И., Пучков В. Ф. и Отеллин В. А. Влияние дефицита серотонина на эмбриональное развитие млекопитающих. Онтогенез, 1995, т. 26, № 5, с. 350–356.
- Brewer G. J., Boehler M. D., Pearson R. A. et al. Neuron network activity scales exponentially with synapse density. J. Neural. Eng., 2009, v. 6, № 1, р. 140–145.
- Crins T. T., Rusu S. I., Rodriguez-Contreras A. and Borst Y. G. Deveplopmental changes in short-term plasticity at the rat calyx of Held synapse. J. Neurosci., 2011, v. 31, № 32, p. 11706–11717.
- Keller H. H. Depletion of cerebral monoamines by p-chlorophenylalanine in the cat. Experientia, 1972, v. 28, № 2, p. 177–178.
- Kim J. E., O’Sullivan M. L., Sanchez C. A. et al. Investigating synapse formation and function using human pluripotent stem cell-derived meurons. Proc. Natl. Acad. Sci. USA, 2011, v. 108, № 7, p. 3005–3010.
- Lauder J. M., Towle A. C. and Patrick K. Decreased serotonin content of embryonic raphe neurons following maternal adminis tration of p-chlorophenylalanine: aquantitative immunocytochemical study. Dev. Brain Res., 1985, v. 20, № 1, p. 107–114.
- Paxinos G. and Watson C. The rat brain in stereotaxic coordinates. Washington, Acad. Press, 2005.