MORPHOMETRIC CHARACTERIZATION OF HEPATOCYTES DURING ADAPTATION TO EXTREME ANTARCTIC FACTORS



Cite item

Full Text

Open Access Open Access
Restricted Access Access granted
Restricted Access Subscription or Fee Access

Abstract

The dynamics of ultrastructural changes in the hepatocytes of albino rats after prolonged influence of extreme Antarctic factors had been studied using the morphometric method. It was found that at the early stage of the experiment (7-45 days) the intracellular homeostasis in the hepatocytes was disturbed owing to a pronounced decrease in energy sources and plastic reserves (the decrease in numerical and volume density of the mitochondria, the reduction of the rough endoplasmic reticulum volume and the dilatation of its cisterns, the increase in the volume of the smooth endoplasmic reticulum). The following stages (up to 180 days) were characterized by a practically complete restoration of energy-plastic potential of the hepatocytes; at the conclusive stage of the experiment (270 days) the exhaustion of energy and, especially, of plastic reserves of the hepatocytes was noted (the significant decline in numerical density of the mitochondria and the relative volume of the rough endoplasmic reticulum).

About the authors

M D SHMERLING

Laboratory of electron microscopy, SB RAMS Scientific Research Institute of Physiology, Novosibirsk

Laboratory of electron microscopy, SB RAMS Scientific Research Institute of Physiology, Novosibirsk

V Sh BELKIN

Department of Anatomy and Anthropology, Tel-Aviv University, Israel

Department of Anatomy and Anthropology, Tel-Aviv University, Israel

Ye Ye FILIUSHINA

Laboratory of electron microscopy, SB RAMS Scientific Research Institute of Physiology, Novosibirsk

Laboratory of electron microscopy, SB RAMS Scientific Research Institute of Physiology, Novosibirsk

O B ASTAKHOV

Department of Human Anatomy, Ulyanovsk State University, Ulyanovsk

Department of Human Anatomy, Ulyanovsk State University, Ulyanovsk

I I BUZUYEVA

Laboratory of electron microscopy, SB RAMS Scientific Research Institute of Physiology, Novosibirsk

Laboratory of electron microscopy, SB RAMS Scientific Research Institute of Physiology, Novosibirsk

V V VERYASKIN

Laboratory of electron microscopy, SB RAMS Scientific Research Institute of Physiology, Novosibirsk

Laboratory of electron microscopy, SB RAMS Scientific Research Institute of Physiology, Novosibirsk

References

  1. Астахов О.Б. Динамика морфометрических показателей яичек крыс при адаптации к высокогорью Антарктиды и последующей дезадаптации. Арх. анат., 1990, т. 99, вып. 12, с. 17-19.
  2. Ващенков В.М., Клонов В.П. и Яковлев В.А. Состояние иммунной системы полярников в Антарктиде. Антарктика, 1995, т. 33, с. 176-180.
  3. Гичев Ю.П. Роль печени в стрессорных реакциях организма. Успехи физиол. наук, 1990, т. 21, № 1, с. 23-46.
  4. Максимов А.Л. и Белкин В.Ш. Характеристика адаптации человека в высокогорье Центральной Азии и Антарктиды. Физиология человека, 2002, т. 28, № 6, с. 6-12.
  5. Молодых О.П., Лушникова Е.Л., Клинникова М.Г. и Непомнящих Л.М. Внутриклеточная реорганизация гепатоцитов при воздействии доксорубицина. Бюл. экспер. биол., 2006, т. 141, № 5, с. 579-585.
  6. Сороко С.И., Матусов А.Л. и Сидоров Ю.А. Адаптация человека к экстремальным условиям Антарктиды. Физиология человека, 1984, т. 10, № 6, с. 907-920.
  7. Шкурупий В.А. Ультраструктура клеток печени при стрессе. Новосибирск, Наука, 1989.
  8. Шмерлинг М.Д., Бузуева И.И., Филюшина Е.Е. и Сагдиева Ш.С. Особенности ультраструктурной реакции мышечных волокон и гепатоцитов адаптированных к холоду животных на острое охлаждение. Бюл. СО РАМН, 1997, № 1, с. 113-117.
  9. Barre H., Bailly L. and Rouanet J. Increased oxidative capacity in skeletal muscles from cold-acclimated ducklings: A comparision with rats. Comp. Biochem. Physiol., 1987, v. 88B, № 2, p. 579-582.
  10. Belkin V., Astakhov O., Buzueva I. et al. Morphophysiological status of albino rats during prolonged stay in the coastal and highland regions of the Antarctic. Baltic J. Lab. Anim. Sci., 2002, v. 12, p. 200-213.
  11. Belkin V. and Karasik D. The effect of season occupation and repeated wintering on anthropologic and physiological characteristics in Russian Antarctic staff. Int. J. Circumpolar Health, 2000, v. 60, p. 41-51.
  12. Farrace S., Ferrara M., De Angelis C. et al. Reduced sympathetic outflow and adrenal secretory activity during a 40-day stay in the Antarctic. Int. J. Psychophysiol., 2003, v. 49, № 1, p. 17-27.
  13. Goglia F., Lanni A., Duchamp C. et al. Effect of cold acclimation on oxidative capacity and respiratory properties of the liver and muscle mitochondria in ducklings. Comp. Biochem. Physiol., 1993, v. 106, № 1, p. 95-101.
  14. Harinath K., Malhotra A., Pal K. et al. Autonomic nervous system and adrenal response to cold in man at Antarcica. Wilderness Environ Med. 2005, v. 16, № 2, p. 81-91.
  15. Sawhney R., Malhotra A., Nair C. et al. Thyroid function during a prolonged stay in Antarctica. Eur. J. Appl. Physiol. Occup. Physiol., 1995, v. 72, № 1-2, p. 127-133.
  16. Toth L. and January B. Physiological stabilization of rabbits after shipping. Lab. Anim. Sci., 1990, v. 40, № 4, p. 384-387.
  17. Van Ruiven R., Meijer G.W., Wiersma A. et al. The influence of transportation stress on selected nutritional parameters to establish the necessary minimum period for adaptation in rat feeding studies. Lab. Anim. 1998, v. 32, № 4, p. 446-56.

Supplementary files

Supplementary Files
Action
1. JATS XML
Download

Copyright (c) 1970 Eco-Vector


СМИ зарегистрировано Федеральной службой по надзору в сфере связи, информационных технологий и массовых коммуникаций (Роскомнадзор).
Регистрационный номер и дата принятия решения о регистрации СМИ: № 0110212 от 08.02.1993.

This website uses cookies

You consent to our cookies if you continue to use our website.

About Cookies