ACCIDENTAL THYMIC INVOLUTIONIN THE GROWING ORGANISMUNDER THE EFFECT OF DIFFERENT TYPESOF STRESSORS



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Abstract

Using the quantitative immunohistochemistry, the immunomodulatory effect of different types of stressors (physical and psychoemotional) on the thymus of growing experimental animals was assessed. Sprague-Dawley rats aged 21 day were exposed either to physical (water immersion) or emotional «expectation» stress for 5 hours daily during 7 consecutive days. After the final exposure to stress, animals were sacrificed, thymus was obtained for weighting and was processed for routine histology and immunohistochemistry (CD3, CD8, ED1, PCNA) with subsequent automatic image analysis. The finding obtained have demonstrated severe accidental thymic involution in both groups of experimental animals, which was more prominent under the effect of the physical stressor as compared to «purely» psychoemotional stressor. The major factors of poststress thymic hypoplasia were the increased apoptosis of the cortical thymocytes and inhibition of their proliferation, but not their increased transport to peripheral immune organs.

About the authors

M Yu Kapitonova

S L Kuznetsov

S V Klauchek

Z I Mokhd Ismail

M Ulla

O V Fedorova

M Yu Kapitonova

Volgograd State Medical University

; Volgograd State Medical University

S L Kuznetsov

Moscow I.M. Sechenov State Medical Academy

; Moscow I.M. Sechenov State Medical Academy

S V Klauchek

Volgograd State Medical University

; Volgograd State Medical University

Z I Mohd Ismail

University Sains Malaysia

; University Sains Malaysia

M Ullah

University Sains Malaysia

; University Sains Malaysia

O V Fedorova

Volgograd State Medical University

; Volgograd State Medical University

References

  1. Акмаев И.Г., Волкова О.В., Гриневич В.В. и Ресненко А.Б. Эволюционные аспекты стрессорной реакции. Вестн. РАМН, 2002, № 6, с. 24-27.
  2. Капитонова М.Ю., Уллах M., Аснизам Асари М. и др. Динамика Thy-1 лимфоцитов в иммунных органах растущего организма при хроническом стрессе. Int. J. Immunorehabilitation. 2003, v. 5, № 2, p. 147-148.
  3. Obminska-Mrukowicz B. and Szczypka M. Influences of DTC and zinc supplementation on the cellular response restoration in restrained mice. J. Vet. Sci., 2005, v. 6, № 1, p. 25-32
  4. Padgett D.A. and Glaser R. How stress influences the immune response. Trends Immunol., 2003, v. 24, № 8, p. 444-448.
  5. Palermo-Neto J., Massoco C.O. and de Souza W.R. Effects of physical and psychological stressors on behavior, macrophage activity and Ehrlich tumor growth. Brain Behav. Immun., 2003, v. 17, p. 43-54.
  6. Pijlman F.T., Wolterink G. and van Ree J.M. Cueing unavoidable physical but not emotional stress increases long-term behavioural effects in rats. Behav. Brain Res., 2002, v. 134, № 1-2, p. 393-401.
  7. Polak J.M. and Van Noorden S. Introduction to immunohistochemistry. 3rd edition. Oxford, Bios Scientific Pub., 2003.
  8. Posevitz V., Benyhe S., Duda E. and Borsodi A. Restraint stress and anti-tumor immune response in mice. Acta Biol. Hung., 2003, v. 54, № 2, p. 167-176.
  9. Pruett S.B. Quantitative aspects of stress-induced immunomodulation. Int. Immunopharmacol., 2001, v. 1, № 3, p. 507-520.
  10. Yamamotova A., Starec M., Holecek V. et al. Anticipation of acute stress in isoprenaline-sensitive and - resistant rats: strain and gender differences. Pharmacol. Toxicol., 2000, v. 87, № 4, p. 161-168.

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