Email this article COMPUTER ASSESSMENT OF AN APOPTOTIC PROCESS DYNAMICS IN THE NUCLEI OF PLACENTAL VILLI SYNCYTIOTROPHOBLAST IN PUERPERAS AFTER THE OUTBURST OF HERPES VIRUS INFECTION
- Authors: LUTSENKO MT1
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Affiliations:
- Laboratory of the Mechanisms of Etiopathogenesis and Regenerative Processes of Respiratory Organs in Non-specific Pulmonary Diseases, Far Eastern Center of Respiratory Physiology and Pathology, RAMS SB, Blagoveshchensk
- Issue: Vol 134, No 6 (2008)
- Pages: 79-83
- Section: Articles
- URL: https://j-morphology.com/1026-3543/article/view/402520
- DOI: https://doi.org/10.17816/morph.402520
- ID: 402520
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Abstract
The development of an apoptotic process in the nuclei of syncytiotrophoblast was studied in the placenta of 50 puerperas, who had the outburst of herpes virus infection in the second part of their pregnancy (antibody titre - 1:12800). Control group was represented by the placental material obtained from 20 women with no disease. Apoptosis was demonstrated in paraffin sections of the material fixed in 10% buffered formalin by in situ labeling of DNA fragments (ISEL-method). The assessment was performed by studying 2000 nuclei from 100 terminal villi in the different areas of the histological section within each placenta. The nuclei in sections were analyzed using a Bio Vision computer program, allowing to detect the areas with the different degree of chromatin condensation during the apoptosis development.
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About the authors
M T LUTSENKO
Laboratory of the Mechanisms of Etiopathogenesis and Regenerative Processes of Respiratory Organs in Non-specific Pulmonary Diseases, Far Eastern Center of Respiratory Physiology and Pathology, RAMS SB, BlagoveshchenskLaboratory of the Mechanisms of Etiopathogenesis and Regenerative Processes of Respiratory Organs in Non-specific Pulmonary Diseases, Far Eastern Center of Respiratory Physiology and Pathology, RAMS SB, Blagoveshchensk
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Supplementary files
СМИ зарегистрировано Федеральной службой по надзору в сфере связи, информационных технологий и массовых коммуникаций (Роскомнадзор).