EFFECT OF TOPICAL APPLICATION OF PLATELETRICH BLOOD PLASMA AND HYDROIMPULSIVE SANATION IN THE TREATMENT OF SKIN WOUNDS ON THE REACTION OF SENSORY NEURONS

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Aim, Material and Methods. In an experiment on 300 white male rats evaluated the reactions of neurons of the dorsal root ganglions innervating the regeneration zone of the skin wound of the thigh, with its natural healing and in the case of infection with Staphilococcus aureus sp., using isolated and combined use of platelet-rich blood plasma (PRP) and hydroimpulsive sanation of the wound defect (HIS). Results and Discussion. The appearance of the neuron groups with various features of morphofunctional changes is noted: reversible by dystrophic and compensatory reactions and irreversible by degenerative changes. The introduction, after the HIS treatment, of PRP in the wound defect resulted in increasing the regenerative effects in the neurons already from the 7th day of the experiment and a significant reduction in cell destruction. The introduction of PRP without pretreatment of the wound defect led to a delay in regeneration and increased the number of destructively altered neurons even in comparison with the spontaneous course of the wound process. Using the multivariate correlation analysis evaluated the relationship between indicators of protein-synthesis activity (optical density of protein and RNA), basic morphometric characteristics (area and nuclear-cytoplasmic index) and condition of perineuronal glial environment. Conclusions. The combined use of HIS and PRP had the most harmonious effect and a high positive correlation, which can be regarded as the most adequate combination of methods of regional influence in the purulent form of wound healing process.