RAPID METHOD OF THE AMYLOID STAINING WITH CONGO RED FOR LIGHT AND FLUORESCENCE MICROSCOPY



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详细

The aim of the present study was to optimize the histochemical method of amyloid staining using Congo red. The study was performed on specimens of the myocardium of left ventricle of the heart obtained at autopsy from the patients with amyloidosis of myocardium diagnosed postmortem. It was shown that a positive impact on the quality of the staining of amyloid is provided by a procedure of pre-heating the slides in the liquid, especially at an acidic pH. The staining protocol was developed allowing to obtain preparations characterized by high-contrast staining of amyloid at light microscopic level and by high intensity of its fluorescence. The advantage of the protocol presented is also a significant reduction in the total duration of staining, an increase in stability of the specific staining of amyloid and the absence of nonspecific background staining.

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作者简介

V. Gusel’nikova

RAS Research Institute of Experimental Medicine

Laboratory of Functional Morphology of the Central and Peripheral Nervous System, Department of General and Special Morphology

O. Kirik

RAS Research Institute of Experimental Medicine

Email: olga_kirik@mail.ru
Laboratory of Functional Morphology of the Central and Peripheral Nervous System, Department of General and Special Morphology

Ye. Fyodorova

RAS Research Institute of Experimental Medicine

Laboratory of Functional Morphology of the Central and Peripheral Nervous System, Department of General and Special Morphology

M. Shavlovskiy

RAS Research Institute of Experimental Medicine; I. P.Pavlov First St. Petersburg State Medical University

Laboratory of Functional Morphology of the Central and Peripheral Nervous System, Department of General and Special Morphology; Laboratory of Cardiomyopathies, Institute of Cardiovascular Diseases

A. Gudkova

I. P.Pavlov First St. Petersburg State Medical University

Laboratory of Cardiomyopathies, Institute of Cardiovascular Diseases

D. Korzhevskiy

RAS Research Institute of Experimental Medicine; I. P.Pavlov First St. Petersburg State Medical University

Email: dek2@yandex.ru
Laboratory of Functional Morphology of the Central and Peripheral Nervous System, Department of General and Special Morphology; Laboratory of Cardiomyopathies, Institute of Cardiovascular Diseases

参考

  1. Коржевский Д. Э., Гиляров А. В. Основы гистохимической техники. СПб.: СпецЛит, 2010.
  2. Рыбакова М. Г., Кузнецова И. А., Семернин Е.Н и др. Информативность биопсии слизистой оболочки полости рта для диагностики системного амилоидоза // Арх. пат. 2013. Т. 75, № 5. С. 3-7.
  3. Шавловский М. М. Молекулярные и генетические основы этиопатогенеза амилоидозов // Мед. акад. журн. 2010. Т. 10, № 4. С. 63-81.
  4. Bennhold H. Eine spezifische Amyloidfärbung mit Kongorot // Münch. Med. Wschr. 1922. Bd. 44. S. 1537-1538.
  5. Clement C. G., Truong L. D. An evaluation of Congo red fluorescence for the diagnosis of amyloidosis // Human Pathology. 2014. Vol. 45. P. 1766-1772.
  6. Gillmore J. D., Hawkins P. N. Pathophysiology and treatment of systemic amyloidosis // Nat. Rev. Nephrol. 2013. Vol. 9, № 10. P. 574-586.

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