THE METHOD OF ANALYSIS OF Y-NEURON POPULATIONS IN THE LATERAL GENICULATE BODY OF THE CAT



如何引用文章

全文:

开放存取 开放存取
受限制的访问 ##reader.subscriptionAccessGranted##
受限制的访问 订阅或者付费存取

详细

The paper presents a method of analysis of cell populations that combines the use of normalized spatial coordinates of the neurons with the morphometric criteria of their evaluation. These algorithms were applied to check the heterogeneity of a population of neurons Y-conducting channel in cat at the level of the lateral geniculate body (LGB). As a specific marker of Y-neurons, SMI-32 antibodies were used. Evaluated The dynamics of the distribution of the number of cells and the orientation of their soma within each layer and mediolaterally along the length of LGB dorsal nucleus (LGBDN). Among the SMI-32-positive neurons, the existence of at least two populations was detected differing in number, orientation and distribution of the soma in different layers of LGBDN. The heterogeneity of Y-neuron population in LGBDN detected in this study is consistent with the earlier electrophysiological data. We believe that the described algorithm for neuronal analysis may be successfully applied to study not only LGB, but also other extensive structures of the brain, including those having laminar organization.

全文:

受限制的访问

作者简介

A. Mikhalkin

RAS I. P.Pavlov Institute of Physiology

Email: michalkin@mail.ru

N. Merkulyeva

RAS I. P.Pavlov Institute of Physiology

Email: mer-natalia@yandex.ru

参考

  1. Конюхов А. Л. Руководство к использованию программного комплекса ImajeJ для обработки изображений: Учебное методическое пособие. Томск: ТУСУР, 2012.
  2. Меркульева Н. С., Михалкин А. А., Никитина Н. И. и др. Изменение формирования Y-нейронов зрительной системы кошки в раннем постнатальном онтогенезе под влиянием бинокулярной ритмической световой стимуляции // Морфология. 2014. Т. 145, вып. 1. С. 13-18.
  3. Bickford M. E., Guido W., Godwin D. W. Neurofilament proteins in Y-cells of the cat lateral geniculate nucleus: normal expression and alteration with visual deprivation // J. Neurosci. 1998. Vol. 18, № 16. P. 6549-6557.
  4. Bowling D. B., Wieniawa-Narkiewicz E. The distribution of on-and off-centre X- and Y-like cells in the A layers of the cat’s lateral geniculate nucleus // J. Physiol. 1986. Vol. 375. P. 561-572.
  5. Boyd J. D., Matsubara J. A. Laminar and columnar patterns of geniculocortical projections in the cat: relationship to cytochrome oxidase // J. Comp. Neurol. 1996. Vol. 365, № 4. P. 659-682.
  6. Carden W. B., Guido W., Ziburkus J. et al. A novel means of Y cell identification in the developing lateral geniculate nucleus of the cat // Neurosci. Lett. 2000. Vol. 295, № 1-2. P. 5-8.
  7. Coleman L. A., Friedlander M. J. Postnatal dendritic development of Y-like geniculocortical relay neurons // Int. J. Dev. Neurosci. 2002. Vol. 20. P. 137-159.
  8. Duffy K. R., Slusar J. E. Monocular deprivation provokes alteration of the neuronal cytoskeleton in developing cat lateral geniculate nucleus // Vis. Neurosci. 2009. Vol. 6, № 3. P. 319-328.
  9. Hoffman K. P., Stone J., Sherman S. M. Relay of receptive-field properties in dorsal lateral geniculate nucleus of the cat // J. Neurophysiol. 1972. Vol. 35, № 4. P. 518-531.
  10. Humphrey A. L., Sur M., Uhlrich D. J., Sherman S. M. Termination patterns of individual X- and Y-cell axons in the visual cortex of the cat: projections to area 18, to the 17/18 border region, and to both areas17 and 18 // J. Comp. Neurol. 1985. Vol. 233. P. 190-212.
  11. Kaplan E. The M, P, and K pathways of the primate visual system revisited // The New Visual Neurosciences. London: MIT Press, 2014. P. 215-227.
  12. LeVay S., Ferster D. Relay cell classes in the lateral geniculate nucleus of the cat and the effects of visual deprivation // J. Comp. Neurol. 1977. Vol. 172, № 4. P. 563-584.
  13. Murphy P. C., Duckett S. G., Sillito A. M. Comparison of the laminar distribution of input from areas 17 and 18 of the visual cortex to the lateral geniculate nucleus of the cat // J. Neurosci. 2000. Vol. 20, № 2. P. 845-853.
  14. Sanderson K. J. The projection of the visual field to the lateral geniculate and medial interlaminar nuclei in the cat // J. Comp. Neurol. 1971. Vol. 143, № 1. P. 101-108.
  15. Schneider C. A., Rasband W.S., Eliceiri K. W. NIH Image to ImageJ: 25 years of image analysis // Nat. Methods. 2012. Vol. 9, № 7. P. 671-675.
  16. Stanford L. R., Friedlander M. J., Sherman S. M. Morphological and physiological properties of geniculate W-cells of the cat: a comparison with X- and Y-cells // J. Neurophysiol. 1983. Vol. 50, № 3. P. 582-608.
  17. Yeh C. I., Stoelzel C. R., Alonso J. M. Two different types of Y cells in the cat lateral geniculate nucleus // J. Neurophysiol. 2003. Vol. 90, № 3. P. 1852-1864.

补充文件

附件文件
动作
1. JATS XML
下载

版权所有 © Eco-Vector, 2016


СМИ зарегистрировано Федеральной службой по надзору в сфере связи, информационных технологий и массовых коммуникаций (Роскомнадзор).
Регистрационный номер и дата принятия решения о регистрации СМИ: № 0110212 от 08.02.1993.
##common.cookie##