电邮这篇文章 METHOD OF IMMUNOCYTOCHEMICAL DEMONSTRATION OF CHOLINERGIC NEURONS IN THE CENTRAL NERVOUS SYSTEM OF LABORATORY ANIMALS
- 作者: Korzhevskiy D.E.1, Grigoriyev I.P.1, Kirik O.V.1, Zelenkova N.M.1, Sukhorukova Y.G.1
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隶属关系:
- RAMS North-Western Branch Institute of Experimental Medicine
- 期: 卷 144, 编号 6 (2013)
- 页面: 069-072
- 栏目: Articles
- URL: https://j-morphology.com/1026-3543/article/view/398705
- DOI: https://doi.org/10.17816/morph.398705
- ID: 398705
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详细
A protocol of immunocytochemical demonstration of choline acetyltransferase (ChAT), a key enzyme of acetylcholine synthesis, in paraffin sections of the brain of some laboratory animals, is presented. The method is simple, gives fairly reproducible results and allows for demonstration of ChAT in neurons, nerve fibers, and terminals in preparations of at least three species of laboratory animals including rat, rabbit, and cat. Different kinds of fixation (10% formalin, 4% paraformaldehyde, or zinc-ethanol-formaldehyde) were found suitable for immunocytochemical visualization of ChAT, however, optimal results were obtained with the application of zinc-ethanol-formaldehyde
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作者简介
D. Korzhevskiy
RAMS North-Western Branch Institute of Experimental Medicine
Email: dek@yandex.ru
I. Grigoriyev
RAMS North-Western Branch Institute of Experimental Medicine
O. Kirik
RAMS North-Western Branch Institute of Experimental Medicine
N. Zelenkova
RAMS North-Western Branch Institute of Experimental Medicine
Ye. Sukhorukova
RAMS North-Western Branch Institute of Experimental Medicine
Email: len48@inbox.ru
参考
- Коржевский Д. Э. и Юмкина Е. А. Применение методов демаскирования антигенов на парафиновых срезах головного мозга крысы. Морфология, 2005, т. 127, вып. 2, с. 76–77.
- Отеллин В. А. и Григорьев И. П. Синаптические и несинаптические межнейронные связи как структурно-медиаторная основа функций неостриатума. В кн.: Стриарная система и поведение в норме и патологии. Л., изд. Ин-та физиологии им. И. П. Павлова АН СССР, 1984, с. 138–146.
- Daulatzai M. A. Early stages of pathogenesis in memory impairment during normal senescence and Alzheimer`s disease. J. Alzheimer Dis., 2010, v. 20, № 2, p. 355–367.
- Mechawar N., Cozzari C. and Descarries L. Cholinergic innervation in adult rat cerebral cortex: a quantitative immunocytochemical description. J. Comp. Neurol., 2000, v. 428, № 2, p. 305–318.
- Picciotto M. R., Higley M. J. and Mineur Y. S. Acetylcholine as a neuromodulator : cholinergic signaling shapes nervous system function and behavior. Neuron, 2012, v. 76, № 1, p. 116–129.
- Platt B. and Riedel G. The cholinergic system, EEG and sleep. Behav. Brain Res., 2011, v. 221, № 2, p. 499–504.
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СМИ зарегистрировано Федеральной службой по надзору в сфере связи, информационных технологий и массовых коммуникаций (Роскомнадзор).