Email this article METHOD OF IMMUNOCYTOCHEMICAL DEMONSTRATION OF CHOLINERGIC NEURONS IN THE CENTRAL NERVOUS SYSTEM OF LABORATORY ANIMALS
- Authors: Korzhevskiy D.E.1, Grigoriyev I.P.1, Kirik O.V.1, Zelenkova N.M.1, Sukhorukova Y.G.1
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Affiliations:
- RAMS North-Western Branch Institute of Experimental Medicine
- Issue: Vol 144, No 6 (2013)
- Pages: 069-072
- Section: Articles
- URL: https://j-morphology.com/1026-3543/article/view/398705
- DOI: https://doi.org/10.17816/morph.398705
- ID: 398705
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Abstract
A protocol of immunocytochemical demonstration of choline acetyltransferase (ChAT), a key enzyme of acetylcholine synthesis, in paraffin sections of the brain of some laboratory animals, is presented. The method is simple, gives fairly reproducible results and allows for demonstration of ChAT in neurons, nerve fibers, and terminals in preparations of at least three species of laboratory animals including rat, rabbit, and cat. Different kinds of fixation (10% formalin, 4% paraformaldehyde, or zinc-ethanol-formaldehyde) were found suitable for immunocytochemical visualization of ChAT, however, optimal results were obtained with the application of zinc-ethanol-formaldehyde
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About the authors
D. E. Korzhevskiy
RAMS North-Western Branch Institute of Experimental Medicine
Email: dek@yandex.ru
I. P. Grigoriyev
RAMS North-Western Branch Institute of Experimental Medicine
O. V. Kirik
RAMS North-Western Branch Institute of Experimental Medicine
N. M. Zelenkova
RAMS North-Western Branch Institute of Experimental Medicine
Ye. G. Sukhorukova
RAMS North-Western Branch Institute of Experimental Medicine
Email: len48@inbox.ru
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СМИ зарегистрировано Федеральной службой по надзору в сфере связи, информационных технологий и массовых коммуникаций (Роскомнадзор).