METHOD FOR SIMULTANEOUS VISUALIZATION OF MAST CELLS AND NERVE TERMINALS IN THE RODENT THYMUS



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详细

The purpose of this study was to develop the method for the simultaneous visualization of mast cells (MCs) and nerve terminals, based on generally accepted techniques of histochemical identification of MCs with alcian blue and immunohistochemical detection of synaptophysin. The protocol presented allows simultaneous identification of mast cells and nerve terminals in the sections of paraffin-embedded thymus of laboratory mammals with high selectivity and good reproducibility. The method can be used for both visualization of spatial relationship between MCs and nerve terminals and independent research of the innervation of mammalian internal organs. Zinc-ethanolformaldehyde is recommended as an optimal fixative.

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作者简介

V. Gusel’nikova

St. Petersburg State University

Ye. Sukhorukova

RAMS North-Western Branch Institute of Experimental Medicine

Email: len48@inbox.ru

Ye. Fyodorova

RAMS North-Western Branch Institute of Experimental Medicine

A. Polevshchikov

RAMS North-Western Branch Institute of Experimental Medicine; Far-Eastern Federal University

D. Korzhevskiy

RAMS North-Western Branch Institute of Experimental Medicine; St. Petersburg State University; St. Petersburg State University

Email: dek2@yandex.ru

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