METHOD FOR SIMULTANEOUS VISUALIZATION OF MAST CELLS AND NERVE TERMINALS IN THE RODENT THYMUS



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Abstract

The purpose of this study was to develop the method for the simultaneous visualization of mast cells (MCs) and nerve terminals, based on generally accepted techniques of histochemical identification of MCs with alcian blue and immunohistochemical detection of synaptophysin. The protocol presented allows simultaneous identification of mast cells and nerve terminals in the sections of paraffin-embedded thymus of laboratory mammals with high selectivity and good reproducibility. The method can be used for both visualization of spatial relationship between MCs and nerve terminals and independent research of the innervation of mammalian internal organs. Zinc-ethanolformaldehyde is recommended as an optimal fixative.

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About the authors

V. V. Gusel’nikova

St. Petersburg State University

Ye. G. Sukhorukova

RAMS North-Western Branch Institute of Experimental Medicine

Email: len48@inbox.ru

Ye. A. Fyodorova

RAMS North-Western Branch Institute of Experimental Medicine

A. V. Polevshchikov

RAMS North-Western Branch Institute of Experimental Medicine; Far-Eastern Federal University

D. E. Korzhevskiy

RAMS North-Western Branch Institute of Experimental Medicine; St. Petersburg State University; St. Petersburg State University

Email: dek2@yandex.ru

References

  1. Коржевский Д. Э., Сухорукова Е. Г., Гилерович Е. Г. и др. Преимущества и недостатки цинк-этанол-формальдегида как фиксатора для иммуноцитохимических исследований и конфокальной лазерной микроскопии. Морфология, 2013, т. 143, вып. 2, с. 81-85.
  2. Корнева Е. А. Введение в иммунофизиологию. СПб., ЭЛБИСПб., 2003.
  3. Лилли Р. Патогистологическая техника и практическая гистохимия. М., Мир, 1969.
  4. Патент РФ № 2386137. Покрытие предметных стекол для проведения иммуноцитохимических и гистологических исследований. Д. Э. Коржевский и О. В. Кирик. Заявка от 29.09.2008. Опубл. в БИ, 2010, № 10. (III ч.), с. 621.
  5. Чумасов Е. И., Ворончихин П. А. и Коржевский Д. Э. Иннервация сердечной поперечнополосатой мышечной ткани легочных вен крысы. Морфология, 2011, т. 140, вып. 6, с. 53-55.
  6. Чумасов Е. И., Петрова Е. С. и Коржевский Д. Э. Иннервация сердца крысы. Морфология, 2009, т. 135, вып. 2, с. 33-37.
  7. Чумасов Е. И., Петрова Е. С. и Коржевский Д. Э. Распределение и структурная организация автономных нервных аппаратов в поджелудочной железе крысы (иммуногистохимическое исследование). Морфология, 2011, т. 139, вып. 3, с. 51-58.
  8. Abramchik G. V., Yermakova S. S., Kaliunov V. N. et al. The immu nomodulatory effect of nerve growth factor. J. Neurosci. Res., 1988, v. 19, № 3, p. 349-356.
  9. Artico M., Cavallotti C. and Cavallotti D. Adrenergic nerve fibres and mast cells: correlation in rat thymus. Immunol. Lett., 2002, v. 84, № 1, p. 69-76.
  10. Bauer O. and Razin E. Mast cell-nerve interactions. Physiology, 2000, v. 15, № 5, p. 213-218.
  11. Botchkarev V.A., Eichmuller S., Peters E. M. et al. A simple immu nofluorescence technique for simultaneous visualization of mast cells and nerve fibers reveals selectivity and hair cycle-dependent changes in mast cell-nerve fiber contacts in murine skin. Arch. Dermatol. Res., 1997, v. 289, № 5, p. 292-302.
  12. Cavallotti D., Artico M., Iannetti G. and Cavallotti C. Occurrence of adrenergic nerve fibers in human thymus during immune response. Neurochem. Int., 2002, v. 40, № 3, p. 211-221.
  13. Lorton D., Bellinger D. L., Felten S. Y. and Felten D. L. Substance P innervation of the rat thymus. Peptides, 1990, v. 11, № 6, p. 1269-1275.
  14. Müller S. and Wihe E. Interrelation of peptidergic innervation with mast cells and ED1-positive cells in rat thymus. Brain Behav. Immun., 1991, v. 5, № 1, p. 55-72.
  15. Newson B., Dahlström A., Enerbäck L. and Ahlman H. Suggestive evidence for a direct innervation of mucosal mast cells: An electron microscopic study. Neuroscience, 1983, v. 10, № 2, p. 565-567.
  16. Novothy G. E., Sommerfels H. and Zirbes T. Thymic innervation in the rat: a light and electron microscopical study. J. Comp. Neurol., 1990, v. 302, № 3, p. 552-561.
  17. Stead R. H., Tomioka M., Quinonez G. et al. Intestinal mucosal mast cells in normal and nematode-infected rat intestines are in intimate contact with peptidergic nerves. Proc. Natl. Acad. Sci. USA, 1987, v. 84, № 9, p. 2975-2979.
  18. Vizi E. S., Orsó E., Osipenko O. N. and Elenkov I. J. Neurochemical, electrophysiological and immunocytochemical evidence for a noradrenergic link between the sympathetic nervous system and thymocytes. Neuroscience, 1995, v. 68, № 4, p. 1263-1276.
  19. Williams J. M., Peterson R. G., Shea P.A. et al. Sympathetic in ner vation of murine thymus and spleen: Evidence for a functional link between the nervous and immune systems. Brain Res. Bull., 1981, v. 6, № 1, p. 83-94.

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